Invisorb Fragment CleanUp

Description

The Invisorb® Fragment CleanUp is a combination of different technologies. Using MSB® technology the kit is the ideal tool for ultra fast purification of DNA fragments (80 bp – 30 kb) after PCR and other enzymatic reactions, for the efficient concentration of DNA fragments as well as for the reliable removal of Dye terminators from DNA cycle sequencing reactions. The recovery of PCR product is 80 – 95 % and a washing step is not required. Using the Invisorb® technology the kit is the ideal tool for the efficient extraction of DNA fragments from TAE or TBE agarose gels.

The Invisorb® Fragment CleanUp is a combination of MSB® Spin PCRapace and Invisorb® Spin DNA Extraction Kit.

The Invisorb® Fragment CleanUp provides ultra fast purification and concentration of up to 100 µl PCR-products from 80 bp up to 30 kb and of other enzymatic reaction mixtures, or of linearized pDNA as well as for the purification of DNA-fragments from agarose gels.

For PCR cleanup the MSB® procedure is offered -no commonly used washing steps are necessary. Finally, the DNA fragments will be eluted with low salt buffer or H2O.

For purification of DNA-fragments from agarose gels the Invisorb® procedure is offered. The DNA fragments are bound directly onto the surface of a spin filter column after gel solubilization. The DNA – fragments will be eluted in a low salt buffer after washing.

The extraction protocol as well as all buffers are optimized to provide high yield and purity of the recovered DNA-fragment. The “hands-on time“ necessary for the whole procedure is reduced to a minimum. The purification process will be ready in 5 – 20 minutes. The purified DNA-fragments are ready to use in various downstream applications such as:

○ digestion with restriction enzymes

○ hybridization, labelling, cloning

○ sequencing

○ In vitro Transcription

○ ligation and transformation

○ DNA sequencing

○ amplification, microinjection