Description
Background:
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress.
RFA3 encodes 122 amino acids with molecular mass of 13,816.
Specifications:
Storage: Shipped at 4℃ and store at -20℃.
Reactivity: S. cerevisiae Rfa3 protein.
Immunogen: Recombinant Rfa3 protein
Antibody: Rabbit antiserum added with 0.05% sodium azide.
Applications:
Western blotting (1/2,000 dilution). Not tested for other applications
Data Link: UniProtKB P26755 (RFA3_YEAST), SGD RFA3/overview RFA3 / YJL173C
Reference: This antibody has not been used in publication.
Fig. Detection of endogenous Rfa3 protein in whole cell extract of S. cerevisiae.
The extract of strain BY4741 (20 μg) was separated on SDS-PAGE 10-20% gradient gel. The anti-Rfa1 antibody was used at 1/2,000 dilution. As the second antibody, HRP-conjugated goat anti-rabbit IgG (ab97051) was used at 1/10,000 dilution. Molecular mass is 13.8 kDa.
