20% off from list price for following items:

• Primary Cells and Cell lines (Innoprot brand)
• DNA/RNA Sample Prep Kits (Invitek brand)
• Proteins and Antibodies (Bioacademia brand)
• PCR Enzymes and Reagents (AS ONE brand)

 

Promo period: 12/4/2019 – 1/31/2020

Promo code: AOI20

 

Note:

• This promotion applies to end-user customers only.
• Other restrictions may apply and will be determined by AS ONE INTERNATIONAL, INC.

Save 30% on Validated Molecular Pathology Antibodies

 

For a list of molecular pathology monoclonal antibodies that have been well validated for immunohistochemistry (IHC), we are offering 30% discount off the list price.

 

This promotion is valid immediately through September 30, 2019.

 

This promo is for end users only. Distributors will be considered for bulk orders only.

 

Please email to info@asone-int.com or call 408-638-7415 for orders or inquiries.

 

 

AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.

AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.

AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH₄⁺ buffer system, dNTPs and magnesium chloride.

AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

 

Cat. #	Product Name	Feature
High Fidelity Taq DNA Polymerase
AO211103	High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer	• 5’→3’ DNA polymerase
		• 3’→5’ proofreading exonuclease
		• Higher fidelity than standard Taq DNA Pol
		• Produces blunt-ended fragments
		• Processes <3 kb with extremely high fidelity
Hot Start Taq DNA Polymerase
AO225103	Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers	• 5’→3’ DNA polymerase
		• Heat activated polymerase
		• Misprimed primers do not extend at RT
		• Higher specificity than standard Taq
		• Greater yield than standard Taq
AO230303	Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2	• Ready-to-use 2.0X master mix
		• Simply add primers, template, and water
		• Set up time is significantly reduced
		• Reduce chance of contaminating components
		• Better reproducibility
Taq DNA Polymerase
AO111203	Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free	• 5’→3’ DNA polymerase
AO112203	Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free	• 5’→3’ polymerase exonuclease
AO114103	Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers	• 5′ overhang
AO140303	Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2	• Ready-to-use 2.0X master mix
		• Simply add primers, template, and water
		• Set up time is significantly reduced
		• Reduce chance of contaminating components
		• Better reproducibility

 

 

Learn More

Partial list of PCR Enzymes

Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.

PSP SalivaGene DNA Kit

The PSP® SalivaGene DNA Kit used with the SalivaGene® Stabilization Collection Tubes is a modular system, combining a DNA stabilization solution for collection, stabilization, storage and transportation of saliva or swab material with a very efficient isolation method for high quality DNA.

 

5 Easy Steps from sample to purified DNA!

For the isolation of genomic host DNA, mitochondrial DNA, pathogenic bacterial and eukaryote DNA from swab and saliva samples.

Field Applications: forensic, human-identity, genetic, biosecurity, pathogen, in vitro diagnostic, pharmacogenomics, and microbiome analysis.

 

SalivaGene Sample Collection Tubes

SalivaGene sample collection tubes containing DNA Stabilizer keeps DNA stable for 12 months at RT or –20°C for years! The DNA stabilization buffer preserves the microorganism titer by prelysis of bacteria, inactivating DNases and preventing DNA degradation. 

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1035200200: PSP SalivaGene DNA Kit,  50 purifications

Catalog # 1035200300: PSP SalivaGene DNA Kit,  250 purifications

Catalog # 1035212200: SalivaGene Collection Module II,  50 tubes

Catalog # 1035212300: SalivaGene Collection Module II ,  125 tubes

 

To Order:

Promotion Code: 12% OFF

Email: info@asone-int.com

Phone: 408-638-7415

 

MSB Technology

MSB® (minimal salt binding) technology, the fastest technology for purification of DNA fragments with high recovery rates. The MSB® kits have been designed for extremely efficient purification and concentration of PCR products or of DNA fragments from enzymatic reaction mixtures with only two steps. The DNA fragments adsorb at the silica membrane in the presence of minimal concentrations of non chaotropic salts, while impurities pass through the column. Then the DNA fragments are eluted in a low-salt buffer or water. Therefore a washing step is not required.

 

MSB Spin PCRapace Kit

MSB Spin PCRapace Kit is a powerful tool to clean up and concentration PCR products or DNA fragments. Just two simple steps — binding, elution — from crude sample to pure DNA

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1020220300: MSB Spin PCRapace, 250 purifications

Catalog # 1020220400: MSB Spin PCRapace, 500 purifications

 

Invisorb Technology

Invisorb® technology is the first technology for the extraction of highly purified nucleic acids using non chaotropic binding conditions. Starting from complex biological samples, the method allows binding of nucleic acids to nearly all surfaces, such as membranes, carrier or magnetic particles. In combination with specially modified surfaces a selective binding of different nucleic acid targets can be realized.

 

Invisorb Fragment Cleanup

The Invisorb^® Fragment CleanUp provides a convenient tool for fast and efficient purification of PCR products and DNA fragments.

Invisorb Fragment Cleanup kit combines 2 Stratec technologies in 1 kit – MSB technology for PCR or DNA fragment cleanup. DNA fragments bind to a silica membrane while the contaminants flow through. Target DNA fragments are eluted in a low-salt buffer or water. Invisorb technology is used for purification of DNA fragments from agarose gels, the DNA fragments are bound to the surface of the spin filter column after the gel is solubilized.

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1020300200: Invisorb Fragment cleanup, 50 purifications

Catalog # 1020300300: Invisorb Fragment cleanup, 250 purifications

 

To Order:

Promotion Code: 12% OFF

Email: info@asone-int.com

Phone: 408-638-7415

 

	Cat.#1020220300	Cat.#1020220400	Cat.# 1020300200	Cat.# 1020300300
	MSB Spin PCRapace              250 purifications	MSB Spin PCRapace                500 purifications	Invisorb Fragment Cleanup                      50 purifications	Invisorb Fragment Cleanup                     250 purifications
STARTING MATERIAL
PCR rxn	X	X	X	X
cDNA synthesis	X	X	X	X
Restriction enzyme digests	X	X	X	X
Ligation rxn	X	X	X	X
Cycle sequencing rxn	X	X	X	X
TAE or TBE agarose gel slices			X	X
ADVANTAGES
DNA purification – enzymatic rxns
Convenient, ultrafast, efficient	X	X	X	X
5 minute, 2 step protocol	X	X	X	X
Purify DNA fragments 80 bp > 30 kbp	X	X	X	X
80-95 % recovery rate	X	X	X	X
Concentrated – elute in 10 µl	X	X	X	X
Gel extraction
20 minute protocol			X	X
Up to 300 mg gel slices			X	X
70-90 % recovery rate			X	X
DOWNSTREAM APPS
Restriction digestion	X	X	X	X
Hybridization, labelling, cloning	X	X	X	X
In vitro transcription	X	X	X	X
Ligation, transformation	X	X	X	X
DNA sequencing	X	X	X	X
Amplification, microinjection			X	X

 

LEARN MORE

MSB Spin PCRapace Protocol                       Invisorb Fragment Cleanup Protocol