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Supplier Spotlight: High quality genetic engineering reagents, Toyobo co.,Ltd.

Since it was established in 1882, TOYOBO has been a leading manufacturer of textile products in Japan. Over the years TOYOBO has expanded its product lines into various non-textile markets such as film, plastics, electronics and life science-related businesses based on the core technologies. Today, TOYOBO provides scientists across the world with a wide variety of unique materials and reagent for applications in the field of Enzymology, Molecular biology, and Immunology. 

To learn more about the ThunderBird TM Next Syber® qPCR Mix (released on 9/2020), click PDF to view the product flyer.

 

To learn more about the KOD Syber® qPCR Mix, click PDF to view the product flyer.

 

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

 

To request the samples for testing, please send an email to info@asone-int.com. 

 

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Supplier Spotlight: Silkworm Protein Expression System: KAICO Ltd.

AS ONE INTERNATIONAL, INC. recently added a new supplier, KAICO Ltd., a startup company founded in April 2018 from the Kyushu University in Japan.  There are over 450 inbred strains of silkworm in the University and KAICO Ltd. selected several unique strains of silkworms which could efficiently produce hard-to-express proteins for vaccine development, diagnostic kit development, and various researches. Their product lineup include Norovirus like particle (VLPs) proteins and SARS-CoV-2 (2019-nCoV) spike protein and antibody.

To learn more about the products, click PDF to view the product flyer;

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

To request the samples for testing, please send an email to info@asone-int.com. Please note that the number of samples is limited.

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Supplier Spotlight: Living organisms and wet samples under an electron microscope: NanoSuit ®

As One International, Inc. recently added a new supplier, NanoSuit Inc. NanoSuit® is a technology that applies a biocompatible polymer solution on the surface of a living organism, tissues or cells to form a micro-thin film that suppresses dehydration of the specimen under the vacuum environment inside an electron microscope.

To learn more about the products, click PDF to view the product flyer.
To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415.
To request the samples for testing, please send an email to info@asone-int.com. Please note that the number of samples is very limited.

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Product Introduction: SuperHiFi High Fidelity DNA Polymerases and Master Mixes

October 23, 2019

 

AS ONE INTERNATIONAL, INC. recently added two new products to its premium PCR reagent portfolio: SuperHiFi High Fidelity DNA Polymerases and SuperHiFi Master Mixes.

To learn more about the products, click PDF to view the product flyer;

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

To request the samples for testing, please send an email to info@asone-int.com. 

 

 

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Stable Cell Lines

Download Flyer for Stable Cell Lines (PDF)

 

Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the gene of interest together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development.

Continue reading Stable Cell Lines

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Sample Preparation

Simplifying Nucleic Acid Preparation with STRATEC Technology

Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. What AS ONE International offers is much more than just another alternative for nucleic acid isolation: the proprietary technologies from Stratec Molecular enable higher quality nucleic acid purification in less time.

Continue reading Sample Preparation

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As One’s Most Popular PCR Enzymes

AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.

AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.

AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH4+ buffer system, dNTPs and magnesium chloride.

AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

 

Cat. # Product Name Feature
High Fidelity Taq DNA Polymerase  
AO211103 High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer • 5’→3’ DNA polymerase
• 3’→5’ proofreading exonuclease
• Higher fidelity than standard Taq DNA Pol
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity
Hot Start Taq DNA Polymerase  
AO225103 Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers • 5’→3’ DNA polymerase
• Heat activated polymerase
• Misprimed primers do not extend at RT
• Higher specificity than standard Taq
• Greater yield than standard Taq
AO230303 Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
• Reduce chance of contaminating components
• Better reproducibility
Taq DNA Polymerase  
AO111203 Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free • 5’→3’ DNA polymerase
AO112203 Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free • 5’→3’ polymerase exonuclease
AO114103 Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers • 5′ overhang
AO140303 Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
 • Reduce chance of contaminating components
 • Better reproducibility

 

 

Learn More

Partial list of PCR Enzymes

Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.

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Peptide Microarrays

AS ONE International provides standard and custom peptide microarrays based on the PEPperCHIP® laser printing platform. Potential applications include epitope mapping, immunological research, biomarker discovery and peptide drug development. Due to their proprietary peptide laser printing technology, the high-content PEPperCHIP® microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility. Check out our offerings below.

Continue reading Peptide Microarrays

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New Tools for Angiogenesis Research

Angiogenesis has a key role in both normal physiology and disease pathology. Caltag Medsystems recently launched two new products for angiogenesis studies: GFP V2a Kit and GFP Human Umbilical Vein Endothelial Cells (HUVEC). The US customers can now order these products through AS ONE INTERNATIONAL.

 

GFP V2a Kit – Vasculogenesis to Angiogenesis

Product Code: ZHA-4100

The GFP V2a Kit – Vasculogenesis to Angiogenesis, is a validated, in vitro, human primary cell co-culture model of angiogenesis that allows the dynamic measurement of all phases of the angiogenic process. Tubules formed contain a lumen and resemble the morphology and range of capillary lengths found in microvascular beds in vivo.

 

Angiogenesis Tested GFP Human Umbilical Vein Endothelial Cells (HUVEC)

Product Code: ZHC-2402

These cells have been shown to produce tubules in conjunction with Cellworks Angiogenesis Tested Human Dermal Fibroblasts (ZHC-5102), using Cellworks Angiogenesis Seeding and Growth Medium (ZHA-1960 and ZHA-1970, respectively). They have been validated for use in the manufacture of Cellworks GFP V2a Kits.

 

Click here to see more products for your angiogenesis research.

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Privacy Policy

Privacy Policy

 

AS ONE INTERNATIONAL, INC shall hold onto the personal information of customers, business partners, board members and employees to run the distribution of research reagents, instruments and equipment, nursing and care products as well as other scientific instruments.

Protecting this personal information appropriately shall be our social responsibility.In order to achieve this, we shall comply with a management system that protects personal information,we shall handle all personal information properly and in good faith and we shall carry out corporate activities with a high level of transparency.

 

  • We shall appropriately obtain, use and provide the personal information of customers, business partners, board members and employees taking into consideration our business as a distributor of research reagents,instruments and equipment, nursing and care products as well as other scientific instruments.

 

  • We shall not handle personal information outside of the scope required to achieve the purpose of use that is specified. In addition, we shall take measures to ensure this.

 

  • We shall comply with all laws and other rules related to handling personal information appropriately.

 

  • We shall clarify regulation related to handling personal information and ensure all employees are informed accordingly. In addition, we shall also request that all outsourcing contractors who are contracted for work to handle all personal information appropriately.

 

  • We shall process complaints and inquiries related to personal information in good faith.

 

  • We shall take preventive and corrective measures that are required to avoid any problems with the personal information we hold, such as being leaked, lost or damaged.

 

  • We shall continue to make the necessary improvements to the management system that protects the personal information in order to ensure that the personal information continues to be handled appropriately.

 

Established on August 1, 2018

AS ONE INTERNATIONAL, INC