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New Supplier: High quality genetic engineering reagents, Toyobo co.,Ltd.

Since it was established in 1882, TOYOBO has been a leading manufacturer of textile products in Japan. Over the years TOYOBO has expanded its product lines into various non-textile markets such as film, plastics, electronics and life science-related businesses based on the core technologies. Today, TOYOBO provides scientists across the world with a wide variety of unique materials and reagent for applications in the field of Enzymology, Molecular biology, and Immunology. Information about TOYOBO’s exclusive enzymes and reagents is available on the web site, www.toyobousa.com.

To learn more about the ThunderBird TM Next Syber® qPCR Mix (released on 9/2020), click PDF to view the product flyer.

 

To learn more about the KOD Syber® qPCR Mix, click PDF to view the product flyer.

 

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

 

To request the samples for testing, please send an email to info@asone-int.com. 

 

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New Supplier: Silkworm Protein Expression System: KAICO Ltd.

AS ONE INTERNATIONAL, INC. recently added a new supplier, KAICO Ltd., a startup company founded in April 2018 from the Kyushu University in Japan.  There are over 450 inbred strains of silkworm in the University and KAICO Ltd. selected several unique strains of silkworms which could efficiently produce hard-to-express proteins for vaccine development, diagnostic kit development, and various researches. Their product lineup include Norovirus like particle (VLPs) proteins and SARS-CoV-2 (2019-nCoV) spike protein and antibody.

To learn more about the products, click PDF to view the product flyer;

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

To request the samples for testing, please send an email to info@asone-int.com. Please note that the number of samples is limited.

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New Supplier: Living organisms and wet samples under an electron microscope: NanoSuit ®

As One International, Inc. recently added a new supplier, NanoSuit Inc. NanoSuit® is a technology that applies a biocompatible polymer solution on the surface of a living organism, tissues or cells to form a micro-thin film that suppresses dehydration of the specimen under the vacuum environment inside an electron microscope.

To learn more about the products, click PDF to view the product flyer.
To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415.
To request the samples for testing, please send an email to info@asone-int.com. Please note that the number of samples is very limited.

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Season’s Greetings and Holiday Savings from AS ONE

20% off from list price for following items:

 

Promo period: 12/4/2019 – 1/31/2020

Promo code: AOI20

 

Note:

  • This promotion applies to end-user customers only.
  • Other restrictions may apply and will be determined by AS ONE INTERNATIONAL, INC.
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New Products: SuperHiFi High Fidelity DNA Polymerases and Master Mixes

October 23, 2019

 

AS ONE INTERNATIONAL, INC. recently added two new products to its premium PCR reagent portfolio: SuperHiFi High Fidelity DNA Polymerases and SuperHiFi Master Mixes.

To learn more about the products, click PDF to view the product flyer;

To place orders, click Product Pages or email to info@asone-int.com or call (408) 638 7415;

To request the samples for testing, please send an email to info@asone-int.com. 

 

 

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Stable Cell Lines

Download Flyer for Stable Cell Lines (PDF)

 

Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the gene of interest together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development.

Continue reading Stable Cell Lines

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Sample Preparation

Simplifying Nucleic Acid Preparation with STRATEC Technology

Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. What AS ONE International offers is much more than just another alternative for nucleic acid isolation: the proprietary technologies from Stratec Molecular enable higher quality nucleic acid purification in less time.

Continue reading Sample Preparation

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30% Off on Validated Molecular Pathology Antibodies

Save 30% on Validated Molecular Pathology Antibodies

 

For a list of molecular pathology monoclonal antibodies that have been well validated for immunohistochemistry (IHC), we are offering 30% discount off the list price.

 

This promotion is valid immediately through September 30, 2019.

 

This promo is for end users only. Distributors will be considered for bulk orders only.

 

Please email to info@asone-int.com or call 408-638-7415 for orders or inquiries.

 

 

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As One’s Most Popular PCR Enzymes

AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.

AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.

AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH4+ buffer system, dNTPs and magnesium chloride.

AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

 

Cat. # Product Name Feature
High Fidelity Taq DNA Polymerase  
AO211103 High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer • 5’→3’ DNA polymerase
• 3’→5’ proofreading exonuclease
• Higher fidelity than standard Taq DNA Pol
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity
Hot Start Taq DNA Polymerase  
AO225103 Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers • 5’→3’ DNA polymerase
• Heat activated polymerase
• Misprimed primers do not extend at RT
• Higher specificity than standard Taq
• Greater yield than standard Taq
AO230303 Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
• Reduce chance of contaminating components
• Better reproducibility
Taq DNA Polymerase  
AO111203 Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free • 5’→3’ DNA polymerase
AO112203 Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free • 5’→3’ polymerase exonuclease
AO114103 Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers • 5′ overhang
AO140303 Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
 • Reduce chance of contaminating components
 • Better reproducibility

 

 

Learn More

Partial list of PCR Enzymes

Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.

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Save 20% on esRAGE ELISA

Save 20% on Human esRAGE ELISA Kits

 

esRAGE is a potential biomarker for metabolic syndrome and cardiovascular diseases. Accurate quantification of esRAGE level is vital to investigate its physiological and pathological roles. AS ONE INTERNATIONAL‘s human esRAGE ELISA kits (formerly under B-Bridge) determines human esRAGE in serum, plasma, or culture medium, and provides accurate quantification of esRAGE specifically.

 

Save 20% on Human esRAGE ELISA Kit (K1009-1) now through November 30, 2019. End users only. Distributors may inquiry for bulk orders.