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IHC Detection

AS ONE INTERNATIONAL is proud to offer Histofine IHC (Immunohistochemistry) detection kits for human, mouse, rabbit, and goat primary antibodies. Uniquely, these kits use just the antigen-recognizing Fab fragment (rather than a whole antibody) to detect a primary antibody. This feature minimizes non-specific binding, thereby increasing the signal-to-noise ratio and generating cleaner results.

Histofine High Stain Systems

Histofine High Stain HRP is the Two-Step Polymer Detection System for IHC staining providing more amplified staining intensity compared with conventional one-step polymer detection system. This system is applicable to both of mouse and rabbit primary antibodies and is for formalin-fixed, paraffin-embedded tissue sections. It is the labeled polymer prepared by combining amino acid polymers with multiple molecules of peroxidase and secondary antibody which is reduced to Fab’ fragment. To eliminate background staining, solid-phase adsorption of secondary antibody is conducted with human serum.

Principle and Procedure

Advantages

  • High intensity of staining
  • Low expression level of antigen is detectable
  • No background of endogenous biotin
  • Reduced reaction time and use of primary antibodies

High Stain HRP Competitive product
Human colon cancer tissue stained with CDX-2 rabbit monoclonal antibody and Histofine High Stain HRP and DAB chromogen. Positive reaction is observed in nuclei of sporadic tumor cells.

Histofine High Stain HRP Detection Products

Product Name Catalog No. Size Note
Histofine High Stain HRP, including Solution A and B 414481F 170 slides (17mL) For use with mouse and rabbit primary antibodies
414483F 1,000 slides (6 x 17mL)

Histofine Simple Stain Systems

Histofine is a universal immuno-enzyme polymer that is used instead of a traditional secondary antibody in IHC staining. Histofine is multiple Fab’ fragments bound to several substrate enzymes such as HRP or AP. Unlike conventional IHC staining with one secondary conjugated antibody detects a single primary antibody, Histofine Simple Stains has multiple Fab and substrate enzymes detecting a single primary antibody. The systems use a precisely cleaved secondary antibody conjugated to an amino acid polymer and multiple enzyme molecules.

Two Histofine Simple Stain systems are available from AS ONE International: Histofine Simple Stain MAX PO and Simple Stain AP. Both Simple Stain systems are detection reagents designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining amino acid polymers with multiple molecules of peroxidase (PO) or alkaline phosphatase (AP) and secondary antibody which is reduced to Fab’ fragment. To eliminate background staining, solid-phase adsorption of secondary antibody is conducted with human serum.

Principle and Procedure

 Simple Stain™ MAX PO

Simple Stain™ AP

Advantages

  •  Simplified staining – Cuts down the conventional streptavidin biotin detection system of 5 steps to 3 steps: Primary antibody → Histofine®Simple Stain → Substrate, eliminates the need for blocking and combines enzyme and secondary antibody into one step.
  • High sensitivity – A shorter polymer length allows deeper tissue penetration with significantly more enzymes and secondary antibodies per detection molecule compared to conventional methods.
  • Low background staining – Biotin-free detection system: no background staining of endogenous biotin; Fab’ fragment: no background staining of endogenous Fc receptor.​
Mouse anti-Progesteron Receptor antibody (clone: 1A6) Rabbit anti-S-100 protein antibody Mouse anti-Estrogen Receptor antibody (clone: 1D5)
Human breast cancer (treated with high temperature epitope unmasking method) stained with Histofine Simple Stain MAX PO (M) and DAB chromogen. Nuclear staining of breast cancer cells is observed. Human colon stained with Histofine Simple Stain MAX PO(R) and DAB chromogen. Cytoplasmic staining of nerve cells is observed. Human breast cancer (treated with high temperature epitope unmasking method) stained with Histofine Simple Stain MAX PO (MULTI) and DAB chromogen. Nuclear staining of breast cancer cells is observed.

 

Mouse anti-Muscle Actin (clone: HHF35) Rabbit anti-S-100 protein antibody
Human stomach stained with Histofine Simpel Stain AP(M) and New Fuchsin chromogen. Intense staining of smooth muscle in the walls of blood vessel and muscularis mucosae is observed. Human colon stained with Histofine Simple Stain AP(R) and New Fuchsin chromogen. Cytoplasmic staining of nerve cells scattered in smooth muscle and Auerbach’s plexus is observed.

 

Histofine Simple Stain MAX PO Products

Product Name Catalog No. Size (slides) Volume For use with
Histofine Simple Stain MAX PO (MULTI) 414151F 170 17ml x 1 Mouse and rabbit primary antibodies
414152F 500 17ml x 3
414154F 1500 17ml x 9
Histofine Simple Stain MAX PO (M) 414131F 170 17ml x 1 Mouse primary antibodies
414132F 500 17ml x 3
414134F 1500 17ml x 9
Histofine Simple Stain MAX PO (R) 414141F 170 17ml x 1 Rabbit primary antibodies
414142F 500 17ml x 3
414144F 1500 17ml x 9
Histofine Simple Stain MAX PO (G) 414161F 170 17ml x 1 Goat primary antibodies
414162F 500 17ml x 3

Histofine Simple Stain AP Products

Product Name Catalog No. Size (slides) Volume For use with
Histofine Simple Stain AP (MULTI) 414261F 170 17ml x 1 Mouse and rabbit primary antibodies
414262F 500 17ml x 3
Histofine Simple Stain AP (M) 414241F 170 17ml x 1 Mouse primary antibodies
414242F 500 17ml x 3
Histofine Simple Stain AP (R) 414251F 170 17ml x 1 Rabbit primary antibodies
414252F 500 17ml x 3