20% off from list price for following items:
Promo period: 12/4/2019 – 1/31/2020
Promo code: AOI20
Note:
Download Flyer for Stable Cell Lines (PDF)
Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the gene of interest together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development.
Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. What AS ONE International offers is much more than just another alternative for nucleic acid isolation: the proprietary technologies from Stratec Molecular enable higher quality nucleic acid purification in less time.
Save 30% on Validated Molecular Pathology Antibodies
For a list of molecular pathology monoclonal antibodies that have been well validated for immunohistochemistry (IHC), we are offering 30% discount off the list price.
This promotion is valid immediately through September 30, 2019.
This promo is for end users only. Distributors will be considered for bulk orders only.
Please email to info@asone-int.com or call 408-638-7415 for orders or inquiries.
AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.
AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.
AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH4+ buffer system, dNTPs and magnesium chloride.
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.
| Cat. # | Product Name | Feature |
| High Fidelity Taq DNA Polymerase | ||
| AO211103 | High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer | • 5’→3’ DNA polymerase |
| • 3’→5’ proofreading exonuclease | ||
| • Higher fidelity than standard Taq DNA Pol | ||
| • Produces blunt-ended fragments | ||
| • Processes <3 kb with extremely high fidelity | ||
| Hot Start Taq DNA Polymerase | ||
| AO225103 | Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers | • 5’→3’ DNA polymerase |
| • Heat activated polymerase | ||
| • Misprimed primers do not extend at RT | ||
| • Higher specificity than standard Taq | ||
| • Greater yield than standard Taq | ||
| AO230303 | Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2 | • Ready-to-use 2.0X master mix |
| • Simply add primers, template, and water | ||
| • Set up time is significantly reduced | ||
| • Reduce chance of contaminating components | ||
| • Better reproducibility | ||
| Taq DNA Polymerase | ||
| AO111203 | Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free | • 5’→3’ DNA polymerase |
| AO112203 | Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free | • 5’→3’ polymerase exonuclease |
| AO114103 | Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers | • 5′ overhang |
| AO140303 | Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2 | • Ready-to-use 2.0X master mix |
| • Simply add primers, template, and water | ||
| • Set up time is significantly reduced | ||
| • Reduce chance of contaminating components | ||
| • Better reproducibility | ||
Learn More
Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.
| Save 20% on Human esRAGE ELISA Kits
esRAGE is a potential biomarker for metabolic syndrome and cardiovascular diseases. Accurate quantification of esRAGE level is vital to investigate its physiological and pathological roles. AS ONE INTERNATIONAL‘s human esRAGE ELISA kits (formerly under B-Bridge) determines human esRAGE in serum, plasma, or culture medium, and provides accurate quantification of esRAGE specifically.
Save 20% on Human esRAGE ELISA Kit (K1009-1) now through November 30, 2019. End users only. Distributors may inquiry for bulk orders.
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AS ONE International provides standard and custom peptide microarrays based on the PEPperCHIP® laser printing platform. Potential applications include epitope mapping, immunological research, biomarker discovery and peptide drug development. Due to their proprietary peptide laser printing technology, the high-content PEPperCHIP® microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility. Check out our offerings below.
PSP SalivaGene DNA Kit
The PSP® SalivaGene DNA Kit used with the SalivaGene® Stabilization Collection Tubes is a modular system, combining a DNA stabilization solution for collection, stabilization, storage and transportation of saliva or swab material with a very efficient isolation method for high quality DNA.
5 Easy Steps from sample to purified DNA!
For the isolation of genomic host DNA, mitochondrial DNA, pathogenic bacterial and eukaryote DNA from swab and saliva samples.
Field Applications: forensic, human-identity, genetic, biosecurity, pathogen, in vitro diagnostic, pharmacogenomics, and microbiome analysis.
SalivaGene Sample Collection Tubes
SalivaGene sample collection tubes containing DNA Stabilizer keeps DNA stable for 12 months at RT or –20°C for years! The DNA stabilization buffer preserves the microorganism titer by prelysis of bacteria, inactivating DNases and preventing DNA degradation.
We are offering a 12% discount for the following kits until August 31, 2018.
Catalog # 1035200200: PSP SalivaGene DNA Kit, 50 purifications
Catalog # 1035200300: PSP SalivaGene DNA Kit, 250 purifications
Catalog # 1035212200: SalivaGene Collection Module II, 50 tubes
Catalog # 1035212300: SalivaGene Collection Module II , 125 tubes
To Order:
Promotion Code: 12% OFF
Email: info@asone-int.com
Phone: 408-638-7415
MSB Technology
MSB® (minimal salt binding) technology, the fastest technology for purification of DNA fragments with high recovery rates. The MSB® kits have been designed for extremely efficient purification and concentration of PCR products or of DNA fragments from enzymatic reaction mixtures with only two steps. The DNA fragments adsorb at the silica membrane in the presence of minimal concentrations of non chaotropic salts, while impurities pass through the column. Then the DNA fragments are eluted in a low-salt buffer or water. Therefore a washing step is not required.
MSB Spin PCRapace Kit
MSB Spin PCRapace Kit is a powerful tool to clean up and concentration PCR products or DNA fragments. Just two simple steps — binding, elution — from crude sample to pure DNA
We are offering a 12% discount for the following kits until August 31, 2018.
Catalog # 1020220300: MSB Spin PCRapace, 250 purifications
Catalog # 1020220400: MSB Spin PCRapace, 500 purifications
Invisorb Technology
Invisorb® technology is the first technology for the extraction of highly purified nucleic acids using non chaotropic binding conditions. Starting from complex biological samples, the method allows binding of nucleic acids to nearly all surfaces, such as membranes, carrier or magnetic particles. In combination with specially modified surfaces a selective binding of different nucleic acid targets can be realized.
Invisorb Fragment Cleanup
The Invisorb® Fragment CleanUp provides a convenient tool for fast and efficient purification of PCR products and DNA fragments.
Invisorb Fragment Cleanup kit combines 2 Stratec technologies in 1 kit – MSB technology for PCR or DNA fragment cleanup. DNA fragments bind to a silica membrane while the contaminants flow through. Target DNA fragments are eluted in a low-salt buffer or water. Invisorb technology is used for purification of DNA fragments from agarose gels, the DNA fragments are bound to the surface of the spin filter column after the gel is solubilized.
We are offering a 12% discount for the following kits until August 31, 2018.
Catalog # 1020300200: Invisorb Fragment cleanup, 50 purifications
Catalog # 1020300300: Invisorb Fragment cleanup, 250 purifications
To Order:
Promotion Code: 12% OFF
Email: info@asone-int.com
Phone: 408-638-7415
| Cat.#1020220300 | Cat.#1020220400 | Cat.# 1020300200 | Cat.# 1020300300 | |
| MSB Spin PCRapace 250 purifications | MSB Spin PCRapace 500 purifications | Invisorb Fragment Cleanup 50 purifications | Invisorb Fragment Cleanup 250 purifications | |
| STARTING MATERIAL | ||||
| PCR rxn | X | X | X | X |
| cDNA synthesis | X | X | X | X |
| Restriction enzyme digests | X | X | X | X |
| Ligation rxn | X | X | X | X |
| Cycle sequencing rxn | X | X | X | X |
| TAE or TBE agarose gel slices | X | X | ||
| ADVANTAGES | ||||
| DNA purification – enzymatic rxns | ||||
| Convenient, ultrafast, efficient | X | X | X | X |
| 5 minute, 2 step protocol | X | X | X | X |
| Purify DNA fragments 80 bp > 30 kbp | X | X | X | X |
| 80-95 % recovery rate | X | X | X | X |
| Concentrated – elute in 10 µl | X | X | X | X |
| Gel extraction | ||||
| 20 minute protocol | X | X | ||
| Up to 300 mg gel slices | X | X | ||
| 70-90 % recovery rate | X | X | ||
| DOWNSTREAM APPS | ||||
| Restriction digestion | X | X | X | X |
| Hybridization, labelling, cloning | X | X | X | X |
| In vitro transcription | X | X | X | X |
| Ligation, transformation | X | X | X | X |
| DNA sequencing | X | X | X | X |
| Amplification, microinjection | X | X |
LEARN MORE
MSB Spin PCRapace Protocol Invisorb Fragment Cleanup Protocol
PSP Spin Stool DNA Plus Kit
The PSP® Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Stool Collection Tube with DNA Stabilizer stabilizes DNA at ambient temperature allowing easy transportation and storage of stool samples. The purification kit is designed for total DNA isolation from microorganisms and from the host organism. The kit can also be used to extract nucleic acids from food and feed residues of plant or animal origin from a stool sample.
Applications:
PCR, RFLP, Hybridization, Genetic typing, Pathogen typing, Mutation analysis, Paternity testing
Using STRATEC Molecular’s unique and proprietary non-chaotropic chemistry, the PSP® (Pre-analytical Sample Processing) product portfolio comprises an integrated sample management system: from sample collection, DNA stabilization, transportation and storage through to purification – all conveniently combined in a single kit.
We are offering a 12% discount for the following kits until August 31, 2018.
Catalog # 1038110200: PSP Spin Stool DNA Plus Kit, 50 purifications
Catalog # 1038110300: PSP Spin Stool DNA Plus Kit, 250 purifications
Additional Stool Collection Tubes with Stabilizer:
Catalog # 1038111200 Catalog # 1038111300: 50 Tubes 250 Tubes
To Order:
Promotion Code: 12% OFF
Email: info@asone-int.com
Phone: 408-638-7415
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