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50% Off on Validated Molecular Pathology Antibodies

Save 50% on Validated Molecular Pathology Antibodies

 

For a list of molecular pathology monoclonal antibodies that have been well validated for immunohistochemistry (IHC), we are offering 50% discount off the list price.

 

This promotion is valid immediately through the end of the year of 2018.

 

This promo is for end users only. Distributors will be considered for bulk orders only.

 

Please email to info@asone-int.com or call 408-638-7415 for orders or inquiries.

 

 

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Stable Cell Lines

Download Flyer for Stable Cell Lines (PDF)

 

Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the gene of interest together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development.

Continue reading Stable Cell Lines

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Sample Preparation

Simplifying Nucleic Acid Preparation with STRATEC Technology

Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. What AS ONE International offers is much more than just another alternative for nucleic acid isolation: the proprietary technologies from Stratec Molecular enable higher quality nucleic acid purification in less time.

Continue reading Sample Preparation

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Peptide Microarrays

AS ONE International provides standard and custom peptide microarrays based on the PEPperCHIP® laser printing platform. Potential applications include epitope mapping, immunological research, biomarker discovery and peptide drug development. Due to their proprietary peptide laser printing technology, the high-content PEPperCHIP® microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility. Check out our offerings below.

Continue reading Peptide Microarrays

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As One’s Most Popular PCR Enzymes

AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.

AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.

AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH4+ buffer system, dNTPs and magnesium chloride.

AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

 

Cat. # Product Name Feature
High Fidelity Taq DNA Polymerase  
AO211103 High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer • 5’→3’ DNA polymerase
• 3’→5’ proofreading exonuclease
• Higher fidelity than standard Taq DNA Pol
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity
Hot Start Taq DNA Polymerase  
AO225103 Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers • 5’→3’ DNA polymerase
• Heat activated polymerase
• Misprimed primers do not extend at RT
• Higher specificity than standard Taq
• Greater yield than standard Taq
AO230303 Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
• Reduce chance of contaminating components
• Better reproducibility
Taq DNA Polymerase  
AO111203 Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free • 5’→3’ DNA polymerase
AO112203 Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free • 5’→3’ polymerase exonuclease
AO114103 Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers • 5′ overhang
AO140303 Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
 • Reduce chance of contaminating components
 • Better reproducibility

 

Learn More

Partial list of PCR Enzymes

Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.

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PSP® SalivaGene DNA Kit

PSP SalivaGene DNA Kit

The PSP® SalivaGene DNA Kit used with the SalivaGene® Stabilization Collection Tubes is a modular system, combining a DNA stabilization solution for collection, stabilization, storage and transportation of saliva or swab material with a very efficient isolation method for high quality DNA.

 

5 Easy Steps from sample to purified DNA!

For the isolation of genomic host DNA, mitochondrial DNA, pathogenic bacterial and eukaryote DNA from swab and saliva samples.

Field Applications: forensic, human-identity, genetic, biosecurity, pathogen, in vitro diagnostic, pharmacogenomics, and microbiome analysis.

 

SalivaGene Sample Collection Tubes

SalivaGene sample collection tubes containing DNA Stabilizer keeps DNA stable for 12 months at RT or –20°C for years! The DNA stabilization buffer preserves the microorganism titer by prelysis of bacteria, inactivating DNases and preventing DNA degradation. 

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1035200200: PSP SalivaGene DNA Kit,  50 purifications

Catalog # 1035200300: PSP SalivaGene DNA Kit,  250 purifications

Catalog # 1035212200: SalivaGene Collection Module II,  50 tubes

Catalog # 1035212300: SalivaGene Collection Module II ,  125 tubes

 

To Order:

Promotion Code: 12% OFF

Email: info@asone-int.com

Phone: 408-638-7415

 

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Purification Kits for PCR Products & DNA Fragments

MSB Technology

MSB® (minimal salt binding) technology, the fastest technology for purification of DNA fragments with high recovery rates. The MSB® kits have been designed for extremely efficient purification and concentration of PCR products or of DNA fragments from enzymatic reaction mixtures with only two steps. The DNA fragments adsorb at the silica membrane in the presence of minimal concentrations of non chaotropic salts, while impurities pass through the column. Then the DNA fragments are eluted in a low-salt buffer or water. Therefore a washing step is not required.

 

MSB Spin PCRapace Kit

MSB Spin PCRapace Kit is a powerful tool to clean up and concentration PCR products or DNA fragments. Just two simple steps — binding, elution — from crude sample to pure DNA

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1020220300: MSB Spin PCRapace, 250 purifications

Catalog # 1020220400: MSB Spin PCRapace, 500 purifications

 

Invisorb Technology

Invisorb® technology is the first technology for the extraction of highly purified nucleic acids using non chaotropic binding conditions. Starting from complex biological samples, the method allows binding of nucleic acids to nearly all surfaces, such as membranes, carrier or magnetic particles. In combination with specially modified surfaces a selective binding of different nucleic acid targets can be realized.

 

Invisorb Fragment Cleanup

The Invisorb® Fragment CleanUp provides a convenient tool for fast and efficient purification of PCR products and DNA fragments.

Invisorb Fragment Cleanup kit combines 2 Stratec technologies in 1 kit – MSB technology for PCR or DNA fragment cleanup. DNA fragments bind to a silica membrane while the contaminants flow through. Target DNA fragments are eluted in a low-salt buffer or water. Invisorb technology is used for purification of DNA fragments from agarose gels, the DNA fragments are bound to the surface of the spin filter column after the gel is solubilized.

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1020300200: Invisorb Fragment cleanup, 50 purifications

Catalog # 1020300300: Invisorb Fragment cleanup, 250 purifications

 

To Order:

Promotion Code: 12% OFF

Email: info@asone-int.com

Phone: 408-638-7415

 

Cat.#1020220300 Cat.#1020220400 Cat.# 1020300200 Cat.# 1020300300
MSB Spin PCRapace              250 purifications MSB Spin PCRapace                500 purifications Invisorb Fragment Cleanup                      50 purifications Invisorb Fragment Cleanup                     250 purifications
STARTING MATERIAL
PCR rxn X X X X
cDNA synthesis X X X X
Restriction enzyme digests X X X X
Ligation rxn X X X X
Cycle sequencing rxn X X X X
TAE or TBE agarose gel slices X X
ADVANTAGES
DNA purification – enzymatic rxns
Convenient, ultrafast, efficient X X X X
5 minute, 2 step protocol X X X X
Purify DNA fragments 80 bp > 30 kbp X X X X
80-95 % recovery rate X X X X
Concentrated – elute in 10 µl X X X X
Gel extraction
20 minute protocol X X
Up to 300 mg gel slices X X
      70-90 % recovery rate X X
DOWNSTREAM APPS
Restriction digestion X X X X
Hybridization, labelling, cloning X X X X
In vitro transcription X X X X
Ligation, transformation X X X X
DNA sequencing X X X X
Amplification, microinjection X X

 

LEARN MORE

MSB Spin PCRapace Protocol                       Invisorb Fragment Cleanup Protocol

 

 

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PSP® Spin Stool DNA Plus Kit

PSP Spin Stool DNA Plus Kit

The PSP® Spin Stool DNA Plus Kit is an integrated system for collection, transportation and storage of stool samples and subsequent DNA purification. Stool Collection Tube with DNA Stabilizer stabilizes DNA at ambient temperature allowing easy transportation and storage of stool samples. The purification kit is designed for total DNA isolation from microorganisms and from the host organism.  The kit  can also be used to extract nucleic acids from food and feed residues of plant or animal origin from a stool sample. 

 

Applications:

PCR, RFLP, Hybridization, Genetic typing, Pathogen typing, Mutation analysis, Paternity testing

 

Using STRATEC Molecular’s unique and proprietary non-chaotropic chemistry, the PSP® (Pre-analytical Sample Processing) product portfolio comprises an integrated sample management system: from sample collection, DNA stabilization, transportation and storage through to purification – all conveniently combined in a single kit.

 

We are offering a 12% discount for the following kits until August 31, 2018.

Catalog # 1038110200: PSP Spin Stool DNA Plus Kit,  50 purifications

Catalog # 1038110300: PSP Spin Stool DNA Plus Kit,  250 purifications

Additional Stool Collection Tubes with Stabilizer:

Catalog # 1038111200 Catalog # 1038111300: 50 Tubes 250 Tubes

 

To Order:

Promotion Code: 12% OFF

Email: info@asone-int.com

Phone: 408-638-7415

 

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BOGO: Immune Checkpoint Antibodies & ELISA Kits

Buy One Get One Free

BIG Savings, BIG Selection

Promo Code: AACR18

 

Immune Checkpoint Product Flyer (PDF)

 

Promotion Details:

 

Antibodies

1) Buy a 100ul size antibody, get a free 30ul size at your choice from 11,000+ primary antibodies

2) Buy a 200ul size antibody, get a free 100ul size at your choice from 11,000+ primary antibodies

 

ELISA Kits

1) Buy one 96T Immune Checkpoint ELISA kit, get one free 48T ELISA kit at your choice from 6000+

 

  • No limits on the number of orders.

 

  • Promo Period: April 15 – June 30, 2018

 

  • Please inquiry for the antibodies or ELISA kits of your interest before ordering, send emails for all inquiries or orders to info@asone-int.com with promo code AACR18.

 

Note: CD40 antibodies on the flyer are not on this promotion.

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Save Up to 45% on Cell Culture Consumables

Download Promo Flyer

 

Dear Researcher,

 

You spend a lot every day to keep cells growing. Do you know how much you can save from your daily consumption of those cell culture plastics without any quality compromise? 

 

AS ONE is a well-known brand in Japan for all cell culture plastic consumables. AS ONE International has introduced its cell culture plastics into the US to help on every step across your cell culture process with a big saving.

 

For a limited time, save up to 45% on the purchase of cell culture flasks, serological pipettes, pipette tips, centrifuge tubes, and petri dishes.

 

   — Quality guaranteed – rigorously tested and used in Japan for many years

   — Save up to 45% from list price, even more from other major suppliers’ offerings

 

To place an order, please click product names on the table below or contact us at info@asone-int.com or call 408-638-7415.

 

Cat. No. Product Name Pack Size List Price ($) Promo Price ($) Discount List Price from one major supplier ($)
3-1491-01 Aznol Petri Dish JP φ90 x 15mm 500 95.00 60.00 37% 769.00
2-8589-01 Cell Cultivation Flask (Vent Cap) 25cm2 200 162.00 145.00 10% 424.00
2-8589-02 Cell Cultivation Flask (Vent Cap) 75cm2 100 155.00 139.00 10% 359.00
2-8589-03 Cell Cultivation Flask (Vent Cap) 175cm2 40 119.00 107.00 10% 208.00 (30 pieces)
3-6629-01 Violamo(R) Sakura Tip Rack Pack 10μl Natural 960 46.00 35.00 24% 81.00
3-6629-02 Violamo(R) Sakura Tip Rack Pack 200μl Yellow 960 46.00 35.00 24% 81.00
3-6629-03 Violamo(R) Sakura Tip Rack Pack 1000μl Blue 960 52.00 35.00 33% 82.00
2-5237-01 Violamo Pipette II Paper/Plastic 1mL 1000 193.00 115.00 40% 172.00 (800 pieces)
2-5237-03 Violamo Pipette II Paper/Plastic 5mL 200 70.00 42.00 40% 61.00
2-5237-04 Violamo Pipette II Paper/Plastic 10mL 200 76.00 50.00 34% 65.00
2-5237-05 Violamo Pipette II Paper/Plastic 25mL 200 138.00 108.00 22% 125.00
2-5237-06 Violamo Pipette II Paper/Plastic 50mL 100 125.00 96.00 23% 170.00 (90 pieces)
2-8588-01 Cell Culture Multi-Well Plate VTC-P6, 6-Well 50 83.00 75.00 10% 239.00 (75 pieces)
2-8588-02 Cell Culture Multi-Well Plate VTC-P12, 12-Well 50 92.00 83.00 10% 269.00 (75 pieces)
2-8588-03 Cell Culture Multi-Well Plate VTC-P24, 24-Well 50 90.00 81.00 10% 298.00 (75 pieces)
2-8588-04 Cell Culture Multi-Well Plate VTC-P48, 48-Well 50 95.00 86.00 10% 357.00 (75 pieces)
2-8588-05 Cell Culture Multi-Well Plate VTC-P96, 96-Well 100 176.00 159.00 10% 264.00 (50 pieces)
1-3500-01 Violamo Centrifuge Tube VIO-15B 15mL (Bulk Pack) 400 110.00 60.00 45% 248.00 (500 pieces)
1-3500-02 Violamo Centrifuge Tube VIO-50B 50mL (Bulk Pack) 200 73.00 50.00 32% 351.00 (500 pieces)
1-3500-11 Violamo Centrifuge Tube VIO-15R 15mL (Rack Pack) 400 156.00 90.00 42% 256.00 (500 pieces)
1-3500-12 Violamo Centrifuge Tube VIO-50R 50mL (Rack Pack) 144 76.00 45.00 41% 214.00 (300 pieces)

 

 

Check out our Microgravity Device Gravite®, and other Cell Culture products.

 

Do you know you can save even more? Subscribe to us and start saving every month on selected research tools.

 

 

Terms & Conditions: 

  1. This offer is only valid in the United States, while supplies last for orders placed between  January 3rd, 2018 and March 30th, 2018, and only in connection with the products identified above when purchased from AS ONE INTERNATIONAL, Inc..
  2. This offer is for the end-users who conduct research in the United States only. Any distributors are not included in this promotion.
  3. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. Customer acknowledges that this offer may include a discount or other price reduction that must be properly and accurately accounted for and reported by customer in accordance with all federal and state laws.