Category: Promotion

Posted on

Stable Cell Lines

Download Flyer for Stable Cell Lines (PDF)

 

Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the gene of interest together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development.

Continue reading Stable Cell Lines

Posted on

Sample Preparation

Simplifying Nucleic Acid Preparation with STRATEC Technology

Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. What AS ONE International offers is much more than just another alternative for nucleic acid isolation: the proprietary technologies from Stratec Molecular enable higher quality nucleic acid purification in less time.

Continue reading Sample Preparation

Posted on

As One’s Most Popular PCR Enzymes

AS ONE High Fidelity DNA Polymerase is a thermostable enzyme with proofreading ability. The High Fidelity-Taq has 16x greater fidelity than Taq polymerase.

AS ONE Hot Start Taq DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. During setup and the first ramp of thermal cycling, the enzyme is not active so misprimed primers are not extended.

AS ONE 2X Master Mixes conveniently includes Taq polymerase, NH4+ buffer system, dNTPs and magnesium chloride.

AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications.

 

Cat. # Product Name Feature
High Fidelity Taq DNA Polymerase  
AO211103 High Fidelity Taq Pol 2.5 U/ul, 10x Ammonium Buffer • 5’→3’ DNA polymerase
• 3’→5’ proofreading exonuclease
• Higher fidelity than standard Taq DNA Pol
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity
Hot Start Taq DNA Polymerase  
AO225103 Hot Start Taq Pol 5U/ul, 10x Ammonium & 10x Combination Buffers • 5’→3’ DNA polymerase
• Heat activated polymerase
• Misprimed primers do not extend at RT
• Higher specificity than standard Taq
• Greater yield than standard Taq
AO230303 Hot Start Taq Pol, 2x Master Mix I 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
• Reduce chance of contaminating components
• Better reproducibility
Taq DNA Polymerase  
AO111203 Taq DNA Pol 5 U/ul 10x Ammonium Buffer, Mg2+ free • 5’→3’ DNA polymerase
AO112203 Taq DNA Pol 5 U/ul 10x Standard Buffer, Mg2+ free • 5’→3’ polymerase exonuclease
AO114103 Taq DNA Pol 5 U/ul, 10x Ammonium & 10x Standard Buffers • 5′ overhang
AO140303 Taq DNA Pol 2x Master Mix, 1.5 mM MgCl2 • Ready-to-use 2.0X master mix
• Simply add primers, template, and water
• Set up time is significantly reduced
 • Reduce chance of contaminating components
 • Better reproducibility

 

 

Learn More

Partial list of PCR Enzymes

Note: Some PCR enzymes and master mixes are available but not on our website yet, so please contact us if you didn’t find what you need on the list.

Posted on

Peptide Microarrays

AS ONE International provides standard and custom peptide microarrays based on the PEPperCHIP® laser printing platform. Potential applications include epitope mapping, immunological research, biomarker discovery and peptide drug development. Due to their proprietary peptide laser printing technology, the high-content PEPperCHIP® microarrays set a new standard in terms of peptide diversity, signal quality and content flexibility. Check out our offerings below.

Continue reading Peptide Microarrays

Posted on

Gravity Controller Gravite®

AS ONE INTERNATIONAL today announced it released Gravite®, a new device for simulating microgravity and hypergravity, to the global market except Japan. Gravite® is a multi-directional gravity device for simulating microgravity and hyper-gravity. By controlled rotation of two axes, the 3D clinostat minimizes the cumulative gravity vector at the center of the device and makes 10-3 g over time average. Gravite® can also create a hyper-gravity environment of 2-3 g by centrifugal force from one axis rotation.

 

Gravite® is developed by Professor Louis Yuge from Hiroshima University who is also Director of Space Bio-Laboratories Co. in Japan. Gravite® is an ideal tool that provides the simulated microgravity environment with real-time gravity monitoring for biological studies. The NASA Kennedy Space Center has introduced the device for simulated microgravity.

 

Learn more about Gravite®.

Posted on

iP-TEC® Live Transport Device

AS ONE INTERNATIONAL, Inc. today announced the launching of iP-TEC Live Transport Device into the US market. Developed by SANPLATEC Corporation, the long-expected Live Transport Device is expected to provide strong support to promote regenerative medicine and other cellular therapy studies as well.

 

Compared with other available transport ways such as dry ice, the live transport method enables the “ready-to-use” transport of induced pluripotent stem (iPS) cells and other cells without freezing them, which not only reduces the risk that cells may be damaged or destroyed when returning to an active state, but also saves time, labor costs and reagent costs. IP-TEC Live Transport Device can maintain the system temperature at the range of 33°C~36°C for 150 hours and more when outside temperature is about 25°C. The IP-TEC Live Transport Device and all accessories are available for ordering through AS ONE INTERNATIONAL now.

 

Learn more about iP-TEC Live Transport Device.

Posted on

Live Transport Device and Temperature Control Box

In life science industry, live cells or tissues are usually frozen with cryoprotectants and transported with dry ice if they need to be moved from one place to another for a relatively long time. There are several problems during the traditional procedure. When freezing cells or thawing cells after freezing there are always loss of cells, no mention those hours that spent on freezing and thawing cells and selected freezing medium. In some cases, cells are transported live but the stability of transportation environment such as temperature retention and vibration control is poor. SANPLATEC’s iP-TEC Live Transport Device has solved all of these problems and makes live transport possible!

Continue reading Live Transport Device and Temperature Control Box

Posted on

Gravity Controller Gravite®

Watch Video:

How Does Gravite Work?

 

Latest published studies that used Gravite (full-text PDFs are available upon request):

Furukawa T, Tanimoto K, Fukazawa T, Imura T, Kawahara Y, and Yuge L, Simulated microgravity attenuates myogenic differentiation via epigenetic regulations, NPJ Microgravity. 2018; 4: 11.

Otsuka T, Imura T, Nakagawa K, Shrestha L, Takahashi S, Kawahara Y, Sueda T, Kurisu K, Yuge L, Simulated microgravity culture enhances the neuroprotective effects of human cranial bone-derived mesenchymal stem cells in traumatic brain injury. Stem Cells Dev. 2018 Jul 5.

Imura T, Nakagawa K, Kawahara Y, Yuge L, Stem cell culture in microgravity and its application in cell-based therapy. Stem Cells Dev. 2018 Aug 7.

 A Microgravity Simulator that NASA Kennedy Space Center introduced!

Microgravity provides a special environment where cells grow without sedimentation and convection. Several studies have shown that the formation of 3D aggregates after the cells were cultured under microgravity conditions. The 3D multicellular spheroids or tissues represent more physiologically relevant in vivo situations needed for biomedical research and drug development.

Continue reading Gravity Controller Gravite®

Posted on

Cell Stretching Systems

Cell Stretching Systems

Menicon’s ShellPa and ShellPa Pro high-performance mechanical cell strain instruments and cell culture chambers allow researchers to explore the impact of mechanical cell strain on cells in culture. Mechanical stress applied to the cells better simulates a natural dynamic physiological environment.

Use ShellPa and ShellPa Pro to investigate:

  • Bone, muscle, cartilage, ligaments & tendons
  • Cardiovascular system
  • Cell adhesion, division, growth & cytoskeletal rearrangement
  • Stem cell proliferation & differentiation

 

ShellPa Mechanical Cell Strain Instrument

The mechanical stresses are closely related to cell proliferation and differentiation or tissue degeneration and development. Additionally, disease processes such as osteoporosis, cancer metastasis, skin disorders and muscle degeneration share a loss of tissue structure, integrity and mechanical transduction. ShellPa, the mechanical cell stretch system from Menicon Life Science, is an instrument for cell culturing that has been developed to replicate a dynamic environment inside the human body. This system is pneumatically driven by an air compressor. It is expected that cell culturing under the mechanical stress provided by this system can bring more biologically and physiologically realistic cell responses compared to a conventional static cell culturing without mechanical stress.

Features:

  • The stretch frequency and stretch ratio (stretch pattern) are easily controllable.
  • Stretch chambers are made with silicone (polydimethylsiloxane, or PDMS) and can be used with this system after being coated (e.g. fibronectin).
  • Lightweight and compact
  • Fits in standard laboratory incubator
  • Stretch frequency: 1-120 cycle(s)/min
  • Stretch ratio: 2, 4, 5, 6, 8, 10, 12, 15 and 20%
  • Use up to 6 stretch chamber simultaneously
  • Removable stretch chambers holder
  • Controller clearly indicates the accumulated operation time

Download : Brochure for ShellPa System

Recent publications:

  • Gangoda, S., et al. “Pulsatile stretch as a novel modulator of amyloid precursor protein processing and associated inflammatory markers in human cerebral endothelial cells“, Scientific Reports, volume 8, Article number: 1689 (2018)
  • Grave, K., et al. “ANGIOTENSIN-(1-7) IMPROVES ENDOTHELIAL FUNCTION AND THE DEVELOPMENT OF ATHEROSCLEROSIS THROUGH THE MAS RECEPTOR.”Journal of Hypertension 34 (2016): e24.
  • Dalbeni, A., et al. “LYCOPENE INCREASES NITRIC OXIDE BIOAVAILABILITY AND INHIBITS ENDOTHELIAL CELLS MIGRATION.”Journal of Hypertension 34 (2016): e24.
  • Gangoda, S. V. S., et al. “PULSATILE STRETCH ALTERS EXPRESSION AND PROCESSING OF AMYLOID PRECURSOR PROTEIN IN HUMAN CEREBRAL ENDOTHELIAL CELLS.”Journal of Hypertension 34 (2016): e24.
  • Urbanski, K., et al. “PERIVASCULAR T REGULATORY CELLS AND ENDOTHELIAL DYSFUNCTION IN HUMAN ATHEROSCLEROSIS.”Journal of Hypertension 34 (2016): e24-e25.
  • Thal, Serge C., and Winfried Neuhaus. “The blood–brain barrier as a target in traumatic brain injury treatment.”Archives of medical research 8 (2014): 698-710.

ShellPa Cell Strain Products:

Cat # Name Size
STMP Programmable ShellPa Pro mechanical cell strain instrument Main Unit, Controller
NNMS Standard ShellPa mechanical cell strain instrument Main Unit, Controller, Air Compressor
SC4Dea 4cm2 Stretch Chamber Pack of 6 chambers

 

Posted on

IHC Detection

AS ONE INTERNATIONAL is proud to offer Histofine IHC (Immunohistochemistry) detection kits for human, mouse, rabbit, and goat primary antibodies. Uniquely, these kits use just the antigen-recognizing Fab fragment (rather than a whole antibody) to detect a primary antibody. This feature minimizes non-specific binding, thereby increasing the signal-to-noise ratio and generating cleaner results.

Continue reading IHC Detection