Category: Promotion

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BOGO: Immune Checkpoint Antibodies & ELISA Kits

Buy One Get One Free

BIG Savings, BIG Selection

Promo Code: AACR18

 

Immune Checkpoint Product Flyer (PDF)

 

Promotion Details:

 

Antibodies

1) Buy a 100ul size antibody, get a free 30ul size at your choice from 11,000+ primary antibodies

2) Buy a 200ul size antibody, get a free 100ul size at your choice from 11,000+ primary antibodies

 

ELISA Kits

1) Buy one 96T Immune Checkpoint ELISA kit, get one free 48T ELISA kit at your choice from 6000+

 

  • No limits on the number of orders.

 

  • Promo Period: April 15 – June 30, 2018

 

  • Please inquiry for the antibodies or ELISA kits of your interest before ordering, send emails for all inquiries or orders to info@asone-int.com with promo code AACR18.

 

Note: CD40 antibodies on the flyer are not on this promotion.

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Save Up to 45% on Cell Culture Consumables

Download Promo Flyer

 

Dear Researcher,

 

You spend a lot every day to keep cells growing. Do you know how much you can save from your daily consumption of those cell culture plastics without any quality compromise? 

 

AS ONE is a well-known brand in Japan for all cell culture plastic consumables. AS ONE International has introduced its cell culture plastics into the US to help on every step across your cell culture process with a big saving.

 

For a limited time, save up to 45% on the purchase of cell culture flasks, serological pipettes, pipette tips, centrifuge tubes, and petri dishes.

 

   — Quality guaranteed – rigorously tested and used in Japan for many years

   — Save up to 45% from list price, even more from other major suppliers’ offerings

 

To place an order, please click product names on the table below or contact us at info@asone-int.com or call 408-638-7415.

 

Cat. No. Product Name Pack Size List Price ($) Promo Price ($) Discount List Price from one major supplier ($)
3-1491-01 Aznol Petri Dish JP φ90 x 15mm 500 95.00 60.00 37% 769.00
2-8589-01 Cell Cultivation Flask (Vent Cap) 25cm2 200 162.00 145.00 10% 424.00
2-8589-02 Cell Cultivation Flask (Vent Cap) 75cm2 100 155.00 139.00 10% 359.00
2-8589-03 Cell Cultivation Flask (Vent Cap) 175cm2 40 119.00 107.00 10% 208.00 (30 pieces)
3-6629-01 Violamo(R) Sakura Tip Rack Pack 10μl Natural 960 46.00 35.00 24% 81.00
3-6629-02 Violamo(R) Sakura Tip Rack Pack 200μl Yellow 960 46.00 35.00 24% 81.00
3-6629-03 Violamo(R) Sakura Tip Rack Pack 1000μl Blue 960 52.00 35.00 33% 82.00
2-5237-01 Violamo Pipette II Paper/Plastic 1mL 1000 193.00 115.00 40% 172.00 (800 pieces)
2-5237-03 Violamo Pipette II Paper/Plastic 5mL 200 70.00 42.00 40% 61.00
2-5237-04 Violamo Pipette II Paper/Plastic 10mL 200 76.00 50.00 34% 65.00
2-5237-05 Violamo Pipette II Paper/Plastic 25mL 200 138.00 108.00 22% 125.00
2-5237-06 Violamo Pipette II Paper/Plastic 50mL 100 125.00 96.00 23% 170.00 (90 pieces)
2-8588-01 Cell Culture Multi-Well Plate VTC-P6, 6-Well 50 83.00 75.00 10% 239.00 (75 pieces)
2-8588-02 Cell Culture Multi-Well Plate VTC-P12, 12-Well 50 92.00 83.00 10% 269.00 (75 pieces)
2-8588-03 Cell Culture Multi-Well Plate VTC-P24, 24-Well 50 90.00 81.00 10% 298.00 (75 pieces)
2-8588-04 Cell Culture Multi-Well Plate VTC-P48, 48-Well 50 95.00 86.00 10% 357.00 (75 pieces)
2-8588-05 Cell Culture Multi-Well Plate VTC-P96, 96-Well 100 176.00 159.00 10% 264.00 (50 pieces)
1-3500-01 Violamo Centrifuge Tube VIO-15B 15mL (Bulk Pack) 400 110.00 60.00 45% 248.00 (500 pieces)
1-3500-02 Violamo Centrifuge Tube VIO-50B 50mL (Bulk Pack) 200 73.00 50.00 32% 351.00 (500 pieces)
1-3500-11 Violamo Centrifuge Tube VIO-15R 15mL (Rack Pack) 400 156.00 90.00 42% 256.00 (500 pieces)
1-3500-12 Violamo Centrifuge Tube VIO-50R 50mL (Rack Pack) 144 76.00 45.00 41% 214.00 (300 pieces)

 

 

Check out our Microgravity Device Gravite®, and other Cell Culture products.

 

Do you know you can save even more? Subscribe to us and start saving every month on selected research tools.

 

 

Terms & Conditions: 

  1. This offer is only valid in the United States, while supplies last for orders placed between  January 3rd, 2018 and March 30th, 2018, and only in connection with the products identified above when purchased from AS ONE INTERNATIONAL, Inc..
  2. This offer is for the end-users who conduct research in the United States only. Any distributors are not included in this promotion.
  3. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. Customer acknowledges that this offer may include a discount or other price reduction that must be properly and accurately accounted for and reported by customer in accordance with all federal and state laws.
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Gravity Controller Gravite®

AS ONE INTERNATIONAL today announced it released Gravite®, a new device for simulating microgravity and hypergravity, to the global market except Japan. Gravite® is a multi-directional gravity device for simulating microgravity and hyper-gravity. By controlled rotation of two axes, the 3D clinostat minimizes the cumulative gravity vector at the center of the device and makes 10-3 g over time average. Gravite® can also create a hyper-gravity environment of 2-3 g by centrifugal force from one axis rotation.

 

Gravite® is developed by Professor Louis Yuge from Hiroshima University who is also Director of Space Bio-Laboratories Co. in Japan. Gravite® is an ideal tool that provides the simulated microgravity environment with real-time gravity monitoring for biological studies. The NASA Kennedy Space Center has introduced the device for simulated microgravity.

 

Learn more about Gravite®.

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iP-TEC® Live Transport Device

AS ONE INTERNATIONAL, Inc. today announced the launching of iP-TEC Live Transport Device into the US market. Developed by SANPLATEC Corporation, the long-expected Live Transport Device is expected to provide strong support to promote regenerative medicine and other cellular therapy studies as well.

 

Compared with other available transport ways such as dry ice, the live transport method enables the “ready-to-use” transport of induced pluripotent stem (iPS) cells and other cells without freezing them, which not only reduces the risk that cells may be damaged or destroyed when returning to an active state, but also saves time, labor costs and reagent costs. IP-TEC Live Transport Device can maintain the system temperature at the range of 33°C~36°C for 150 hours and more when outside temperature is about 25°C. The IP-TEC Live Transport Device and all accessories are available for ordering through AS ONE INTERNATIONAL now.

 

Learn more about iP-TEC Live Transport Device.

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Live Transport Device and Temperature Control Box

In life science industry, live cells or tissues are usually frozen with cryoprotectants and transported with dry ice if they need to be moved from one place to another for a relatively long time. There are several problems during the traditional procedure. When freezing cells or thawing cells after freezing there are always loss of cells, no mention those hours that spent on freezing and thawing cells and selected freezing medium. In some cases, cells are transported live but the stability of transportation environment such as temperature retention and vibration control is poor. SANPLATEC’s iP-TEC Live Transport Device has solved all of these problems and makes live transport possible!

Continue reading Live Transport Device and Temperature Control Box

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Gravity Controller Gravite®

Watch Video:

How Does Gravite Work?

 

Latest published studies that used Gravite (full-text PDFs are available upon request):

Furukawa T, Tanimoto K, Fukazawa T, Imura T, Kawahara Y, and Yuge L, Simulated microgravity attenuates myogenic differentiation via epigenetic regulations, NPJ Microgravity. 2018; 4: 11.

Otsuka T, Imura T, Nakagawa K, Shrestha L, Takahashi S, Kawahara Y, Sueda T, Kurisu K, Yuge L, Simulated microgravity culture enhances the neuroprotective effects of human cranial bone-derived mesenchymal stem cells in traumatic brain injury. Stem Cells Dev. 2018 Jul 5.

Imura T, Nakagawa K, Kawahara Y, Yuge L, Stem cell culture in microgravity and its application in cell-based therapy. Stem Cells Dev. 2018 Aug 7.

 A Microgravity Simulator that NASA Kennedy Space Center introduced!

Microgravity provides a special environment where cells grow without sedimentation and convection. Several studies have shown that the formation of 3D aggregates after the cells were cultured under microgravity conditions. The 3D multicellular spheroids or tissues represent more physiologically relevant in vivo situations needed for biomedical research and drug development.

Continue reading Gravity Controller Gravite®

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Cell Stretching Systems

Cell Stretching Systems

Menicon’s ShellPa and ShellPa Pro high-performance mechanical cell strain instruments and cell culture chambers allow researchers to explore the impact of mechanical cell strain on cells in culture. Mechanical stress applied to the cells better simulates a natural dynamic physiological environment.

Use ShellPa and ShellPa Pro to investigate:

  • Bone, muscle, cartilage, ligaments & tendons
  • Cardiovascular system
  • Cell adhesion, division, growth & cytoskeletal rearrangement
  • Stem cell proliferation & differentiation

 

ShellPa Mechanical Cell Strain Instrument

The mechanical stresses are closely related to cell proliferation and differentiation or tissue degeneration and development. Additionally, disease processes such as osteoporosis, cancer metastasis, skin disorders and muscle degeneration share a loss of tissue structure, integrity and mechanical transduction. ShellPa, the mechanical cell stretch system from Menicon Life Science, is an instrument for cell culturing that has been developed to replicate a dynamic environment inside the human body. This system is pneumatically driven by an air compressor. It is expected that cell culturing under the mechanical stress provided by this system can bring more biologically and physiologically realistic cell responses compared to a conventional static cell culturing without mechanical stress.

Features:

  • The stretch frequency and stretch ratio (stretch pattern) are easily controllable.
  • Stretch chambers are made with silicone (polydimethylsiloxane, or PDMS) and can be used with this system after being coated (e.g. fibronectin).
  • Lightweight and compact
  • Fits in standard laboratory incubator
  • Stretch frequency: 1-120 cycle(s)/min
  • Stretch ratio: 2, 4, 5, 6, 8, 10, 12, 15 and 20%
  • Use up to 6 stretch chamber simultaneously
  • Removable stretch chambers holder
  • Controller clearly indicates the accumulated operation time

Download : Brochure for ShellPa System

Recent publications:

  • Gangoda, S., et al. “Pulsatile stretch as a novel modulator of amyloid precursor protein processing and associated inflammatory markers in human cerebral endothelial cells“, Scientific Reports, volume 8, Article number: 1689 (2018)
  • Grave, K., et al. “ANGIOTENSIN-(1-7) IMPROVES ENDOTHELIAL FUNCTION AND THE DEVELOPMENT OF ATHEROSCLEROSIS THROUGH THE MAS RECEPTOR.”Journal of Hypertension 34 (2016): e24.
  • Dalbeni, A., et al. “LYCOPENE INCREASES NITRIC OXIDE BIOAVAILABILITY AND INHIBITS ENDOTHELIAL CELLS MIGRATION.”Journal of Hypertension 34 (2016): e24.
  • Gangoda, S. V. S., et al. “PULSATILE STRETCH ALTERS EXPRESSION AND PROCESSING OF AMYLOID PRECURSOR PROTEIN IN HUMAN CEREBRAL ENDOTHELIAL CELLS.”Journal of Hypertension 34 (2016): e24.
  • Urbanski, K., et al. “PERIVASCULAR T REGULATORY CELLS AND ENDOTHELIAL DYSFUNCTION IN HUMAN ATHEROSCLEROSIS.”Journal of Hypertension 34 (2016): e24-e25.
  • Thal, Serge C., and Winfried Neuhaus. “The blood–brain barrier as a target in traumatic brain injury treatment.”Archives of medical research 8 (2014): 698-710.

ShellPa Cell Strain Products:

Cat # Name Size
STMP Programmable ShellPa Pro mechanical cell strain instrument Main Unit, Controller
NNMS Standard ShellPa mechanical cell strain instrument Main Unit, Controller, Air Compressor
SC4Dea 4cm2 Stretch Chamber Pack of 6 chambers

 

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IHC Detection

AS ONE INTERNATIONAL is proud to offer Histofine IHC (Immunohistochemistry) detection kits for human, mouse, rabbit, and goat primary antibodies. Uniquely, these kits use just the antigen-recognizing Fab fragment (rather than a whole antibody) to detect a primary antibody. This feature minimizes non-specific binding, thereby increasing the signal-to-noise ratio and generating cleaner results.

Continue reading IHC Detection

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3-D Cell Culture Hydrogels

AS ONE INTERNATIONAL is proud to bring you two brands of distinct hydrogels for 3-D cell culture: Cellendes, a life science company in Germany, developed a biomimetic dextran-based hydrogel while Menicon Life Science in Japan manufactures a peptide-based hydrogel. Each hydrogel offers unique advantages for a variety applications like drug discovery and tissue engineering.

Continue reading 3-D Cell Culture Hydrogels

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Red/ET Recombineering

Are you tired of finding restriction sites? AS ONE INTERNATIONAL provides high quality and easy-to-use kits and reagents for cloning, sub-cloning, generating transgenic animals, and manipulating DNA constructs. Particularly, Red/ET Recombineering offers unprecedented freedom in gene cloning. It gives you the powerful tools to clone, subclone, and modify DNA at any chosen position. These Red/ET Kits, Selection Cassettes, and Recombinase Plasmids permit precise engineering of DNA molecules of any size, including very large ones such as BACs or the E. coli chromosomes. Continue reading Red/ET Recombineering