The 3-D Life Dextran-CD Hydrogel SG Kit provides reagents for setting up hydrogels for 3-D cell culture or related applications. Its major components are SG-Dextran and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with SH-reactive groups on SG-Dextran. The resulting polymer netwok forms a gel. The crosslinking step can be performed in the presence of cells to embed cells in the hydrogel.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell adhesive matrix.
The formation of hydrogels is performed at physiological pH for optimal cell compatibility. Gelation starts several minutes after mixing the components at room temperature (compare table in General Protocol GP 2), providing enough time to conveniently manipulate the solution before the onset of gel formation.
SG-Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1) to recover chemically fixed or live cells after culture for post-culture analyses (e.g. RT-PCR) or further cultivation.