Each vial of HiTSeeker ADRA1B Assay Cell Line contains U2OS cells stably expressing human Alpha-1B adrenergic Receptor (ADRA1B) with no tag. We offer this cell line as a stable cell line but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate. Innoprot HiTSeeker ADRA1B Assay Cell Line has been designed to assay compounds or analyze their capability to modulate Alpha-1B adrenergic Receptor. When epinephrine binds to the ADRA1B a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (e.g. Calcium). This cell line has been validated measuring calcium increase in the cytosol. The high reproducibility of this assay allows monitoring ADRA1B receptor activation process in High Throughput Screening.