Anti-ADAM1B Antibody, Rat Monoclonal (#57)

$345.00

SKU: 73-010 Research Area: Applications:

Description

Background:

ADAM1B may play a role in spermatogenesis, sperm maturation and fertilization. Molecular mass is 89,369 with 806 amino acids. N-terminal signal peptide with 33 amino acids from this protein is processed to give propeptide, which may undergo further processing.

 

Key words: Acrosome reaction, Membrane fusion, Protein trafficking, IZUMO1, Sperm–egg fusion

 

Specification:

Reactivity: Mouse.

Immunogen: Mouse sperm

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized. Azide- and carrier-free.

Storage: Shipped at 4℃ and store at -20℃

 

Applications:

Immunocytochemistry (1/100 – 1/300)

Immunohistochemistry (1/100~1/300) paraffin-embedded section.

Not suitable for western blotting; recommend to use clone #158 (#73-007 ) for western blotting.

 

Database Links:

UniProtKB Q8R534 (mouse ADAM1b)

 

Reference: This antibody was described in and used in the following publications.

Ikawa M. et al. Calsperinis a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011;286:5639-46. IF

 

Satouh Y., et al (2012) Visualization of the moment of mouse sperm–egg fusion and dynamic localization of IZUMO1. J. Cell Science 125, 4985–4990. PubMed  22946049. IF.

 

 

Fig.1 Immunofluorescent staining of ADAM1B in sperm by using anti-ADAM1B antibody. Fresh sperm collected from the epididymis of mice were fixed in 4% paraformaldehyde and then immunostained with the anti-ADAM1B antibody (#57) at 1/300 dilution.

 

 

Fig.2. Immunohistological staining of ADAM1B in mouse testis using anti-ADAM1B antibody (#57). A section of formalin fixed and paraffin embedded mouse testis was treated with the anti-ADAM1B antibody at 1/100 dilution after deparaffization and antigen retrieval. The 2nd antibody, anti-rat IgG conjugated with AF488 (Abcam) was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merge). The bright field microsopic picture of the same region was shown on the right.

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