anti-At 12S antibody, rabbit polyclonal 200 ug

$430.00

SKU: 81-123 Research Area: Applications: , , ,

Description

Background:

Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond. Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA)

 

Subcellular location: Vacuole

 

Specifications:

Storage: Shipped at 4°C and store at -20℃

Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.

Purity: IgG, affinity-purified with Protein A

Immunogen: A. thaliana 12S globulinα-subunit purified on SDS-PAGE.

Reactivity: Reacts with A thaliana 12S globulin (precursor andα-subunit). Not tested in other species

 

Applications

  1. Western blot (1/3,000- 1/10,000 dilution)
  2. ELISA (assay dependent)
  3. Immunohistochemistry (1/100)
  4. Immunoelectron microscopic analysis (1/50)

Fig. 1 Western Blot of the precursor 12S globulin and 12S-αsubunit of the mature forms in extract of arabidopsis dry seeds.

M: Protein size markers

1: SDS-PAGE of wild-type seed.

2: SDS-PAGE of of atvst1-1 mutant (defective in storage protein transport) seed.

3: Western blot of wild-type seed

4: Western blot of atvst1-1 mutant seed

The anti-12S globulin antibody was used at 1/5,000 dikution.

P12S is the precursor of 12S globulin.

12S-α is the α-subunit of 12S globulin.

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81-123 anti-At 12S antibody application/pdf 404 KB