Description
Background:
Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond. Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA)
Subcellular location: Vacuole
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: A. thaliana 12S globulinα-subunit purified on SDS-PAGE.
Reactivity: Reacts with A thaliana 12S globulin (precursor andα-subunit). Not tested in other species
Applications
- Western blot (1/3,000- 1/10,000 dilution)
- ELISA (assay dependent)
- Immunohistochemistry (1/100)
- Immunoelectron microscopic analysis (1/50)
Fig. 1 Western Blot of the precursor 12S globulin and 12S-αsubunit of the mature forms in extract of arabidopsis dry seeds.
M: Protein size markers
1: SDS-PAGE of wild-type seed.
2: SDS-PAGE of of atvst1-1 mutant (defective in storage protein transport) seed.
3: Western blot of wild-type seed
4: Western blot of atvst1-1 mutant seed
The anti-12S globulin antibody was used at 1/5,000 dikution.
P12S is the precursor of 12S globulin.
12S-α is the α-subunit of 12S globulin.