Description
Background:
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds
Subcellular location: Vacuole
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A/G mix
Immunogen: Synthetic peptide (SIYRTVVEFEEDDASNC) corresponding to the N-terminal propeptide of an Arabidopsis 2S albumin (2S3) and a Cys residue that was used as a linker for conjugation of the peptide to BSA.
Reactivity: Reacts with A thaliana 2S3 albumin precursor but does not react with the processed mature forms. Not tested in other species.
Applications
- Western blot (1/2,500 dilution)
- ELISA (assay dependent)
- Immunoelectron microscopic analysis (1/500 dilution)
Fig. 1 Western Blot of the precursor 2S albumin in extract of arabidopsis dry seeds with anti-2S3P antibody.
Western blot showing that anti-2S3P antibody recognizes 2S albumin precursors (p2S) but not the mature forms (2S). Dry seeds of wildtype and mag2-2 (defective in processing the precursors) plants were subjected to immunoblotting with anti-2S antibody and anti-2S3P antibody (1/5,000 dilution)
