Description
Cdk1 (cyclin-dependent kinase1) plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it. Molecular mass is 34,506.
Specifications:
Reactivity: Xenopus, human
Immunogen: Synthetic peptide corresponding to C-terminal region of Xenopus Cdk1 protein (C-KSSLPDNQIRN) conjugated with KLH
Purity: Purified IgG
Form: 2.0 mg/ml in 1 x PBS and 50% glycerol
Storage: Shipped at 4°C and store at -20℃
Applications
Western blotting (1/200-1/500 dilution)
Immunoprecipitation (1/100 dilution)
Data Link:
UniProtKB P35567 (CDK1A_XENLA)
Reference: This antibody was described in Ref.1 and used in Ref 1 and 2.
Masuda H and Shibata T. Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body. J Cell Sci.1996 Jan;109 ( Pt 1):165-77. WB, IP
Kimura K et al. Phosphorylation and activation of 13S condensin by Cdc2 in vitro. 1998 Oct 16;282(5388):487-90. WB
Figure. Detection of endogenous Cdk1 in Xenopus egg extract by Western blot.
Extract (30 μg) of Xenopus laevis eggs was used for western blot analysis and the antibody was used at 1/200 dilution. The molecular mass is 34.5 kDa
