Description
Background:
Cytochrome f is a component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 4 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: Recombinant Spinach Cytochrome f expressed in E. coli.
Reactivity: Cytochrome f of plant including those of maize, Arabidopsis, and spinach
Applications
- Western blot (1/1,000- 1/5,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of Cyt f in plant leaf extract.
Anti-Cyt f antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 20 μg
- Maize leaf extract, 10 μg
Molecular masses of maize Cyt f is 35 kDa (Arabidopsis), 36 kDa (maize). The differences between the predicted sizes and Western Blot data reflect signal peptide removal in mature proteins.
