Anti-DDX18 Antibody, Rabbit Serum

Description

DDX18 protein (670aa, 75.4KDa) is a putative ATP-dependent RNA helicase since it possesses a DEAD box motif, characterized by the conserved motif, Asp-Glu-Ala-Asp (DEAD).RNA helicases which may play a role in ribosome biogenesis. DDX18 acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It was also involved in HIV-1 replication and interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

 

Applications

Western blot (1000~ 2,000 fold dilution)

Immunofluorescence staining (~500 fold dilution)

Not tested for other applications

 

Specification

Reactivity: Reacts with DDX18 of human and rodents.

Immunogen: Purified Human DDX18 fused with GST

Form: Undiluted anti rabbit serum added with 0.05 % sodium azide

Storage: Shipped at 4°C Upon arrival aliquot and store at -20°C

 

Data Link 

UniProtKB/Swiss-Prot Q9NVP1 (DDX18_HUMAN)

 

Reference  This product is described and used in reference 1.

 

Sekiguchi T et al “NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.” Nucleic. Acid. Res. 34 : 4593-4606 (2006) PMID: 16963496

 

 

Fig.1 Western blot analysis of DDX3 in the whole cell extracts of HeLa cells (10g) with anti -DDX18 antibody at 1,000 fold dilution

 

Fig.2  Immunostaining of DDX18 in HeLa cells with anti DDX18 antibody followed by FITC-conjugated anti-rabbit IgG second antibody.DNA was stained with Hoechst dye.