Anti-Diphtheria Toxin Antibody (Rabbit Serum)

Description

Diphtheria toxin (535 aa, 58 kDa), is an exotoxin secreted as a proenzyme by Corynebacterium diphtheriae, the pathogen bacterium that causes diphtheria. The toxin gene is encoded by a bacteriophage. The proenzyme undergoes proteolytic nicking and reduction of disulfide bond, giving rise to subunit A (21 kDa) and B (37 kDa). Subunit B (C-terminal) recognizes cellular receptor, HB-EGF receptor, and subunit A (N-terminal) catalyzes the ADP-ribosylation of eukaryotic elongation factor-2 (eEF2), inactivating this protein.

 

Applications

Western blot (1/2,000 dilution)

ELISA (1/2,000 ~ 1/7,000 dilution. Detected 1 ng or less of the toxin)

Immunoprecipitation (1/200 ~ 1/1,000 dilution, depends on assay)

Dot blot (1/1,000 dilution. Detected 1 ng or less of the toxin)

 

Specification

Immunogen: Purified nontoxic mutant Diphtheria toxin CRM 197 (Ref. 1, Product #01-515)

Specificity: React with Diphtheria toxin as well as its CRM197 mutant toxin

Form: Whole antiserum sterilized by filtration.

Storage: Shipped at 4°C and store at -20°C or below

 

Data Link: 

UniProt KB Diphtheria

 

References:

Uchida T. et al. “Diphtheria toxin and related proteins. Reconstitution of hybrid “diphtheria toxin” from nontoxic Mutant proteins.” J. Biol. Chem. 248:3851-3854 (1973) PMID: 4196586

 

Pappenheimer A. “Diphtheria toxin.”Annu Rev Biochem 46: 69-94 (1977) PMID: 2004

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 1 (left):  Silver Staining, SDS-PAGE analysis of culture medium of Corynebacterium Diphtheria. Lane 1: Protein size markers (kDa). Lane 2: Purified Diphtheria CRM197 mutant protein. Lane 3: Purified Wild-type Diphtheria toxin. Lane 4: Culture medium of Corynebacterium Diphtheria.

Figure 2 (right):  Western blot of culture medium of Corynebacterium Diphtheria. Lane 1: Protein size markers (kDa). Lane 2 (*1): Crude culture medium of Corynebacterium Diphtheria. Lane 3 (*2): The two-fold diluted culture medium. Lane 4: Purified CRM197 (10 ng). Lane 5: Purified Diphtheria toxin (200 ng). Western blotted with this antibody at a 1/2000 dilution.