Anti-Dis3 (S. pombe) Antibody, Rabbit Serum

Description

S.pombe Dis3 protein is an essential component for mitotic segregation (ref.1). It is a component of the exosome 3′->5′ exoribonuclease complex. It is required for the 3′-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran and has a 3′-5′ exonuclease activity. It is composed of 970 amino acids with molecular mass of 110 kDa. It is highly conserved functionally and structurally from yeast to human.

Applications

Western blotting (100~300 fold dilution)

Immunofluorescence staining

Specification

Immunogen: Recombinant truncated Dis3 protein (70 kDa)

Specificity: Reacts with S. pombe Dis3 protein. Not tested for other species.

Form: Rabbit antiserum with 0.05% sodium azide, 50% glycerol

Storage: Shipped at 4°C or -20°C, upon arrival aliquot and store at -20°C

Reference

Kinoshita N “The fission yeast dis3 gene encodes a 110-kDa essential protein implicated in mitotic control” Mol Cell Biol 11:5839-5847(1991) PMID: 1944266

Noguchi E et al “Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1” EMBO J 15:5595-5605(1996) PMID: 8896453

Fig.1 Immunoblotting of extracts of S. pombe cells transformed with the vector or plasmids carrying truncated genes (172, A, B, C, E) with anti-dis3 antibodies. Polipeptides of expected molecular masses were detected (ref.1)

Fig.2 Localization of the dis3 gene product by immunofluorescence microscopy. S. pombe cells were fixed and prepared for immunofluorescence microscopy with anti-dis3 antibodies. Left, DAPI stain for chromosomal DNA. Right, anti-dis3 antibody stain (ref.1).