Description
Background:
Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 in Arabidopsis. Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria. Ferredoxin-dependent glutamate synthase, chloroplastic (Fd-GOGAT) is involved in glutamate biosynthesis in leaf. This protein required for the reassimilation of ammonium ions generated during photorespiration. Gene name is GlsF.
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A/G mix
Immunogen: Purified recombinant maize Fd-GOGAT protein (full size, no tag attached)
Reactivity: Fd-GOGAT including those of maize, Arabidopsis, spinach, and cyanobacterium (Synechococcus)
Validation: Specificity has been validated by Western Blot with recombinant full-size maize Fd-GOGAT protein.
Applications
- Western blot (1/2,000- 1/5,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of Fd-GOGAT in plant leaf extract.
- Full-size recombinant maize Fd-GOGAT protein
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg
Anti-Fd-GOGAT antibody was used at 1/2,500 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
Molecular masses of maize and arabidopsis Fd-GOGAT are 175 kDa and 168 kDa, respectively.
Fig. 2 Western Blot of Fd-GOGAT in cyanobacterium
Sample: Crude extract of Synechococcus spp.6803
The Fd-GOGAT antibody was used at 1/2000 dilutions
Molecular mass is 169 kDa
