Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Fd3 is non-photosynthetic Fd expressed more in root than in leaf.
Subcellular location: Chloroplast and Plastid
Specifications:
Storage: Shipped at 4°C and store at -20℃. (Do not freeze below -25˚C)
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: Purified recombinant maize leaf Fd3 protein (full size, no tag attached)
Reactivity: Plant Fd3 proteins including that of maize and Arabidopsis. Cross-reacts weakly with other Ferredoxin isoproteins, like Arabidopsis and Maize Fd2, and Maize Fd6.
Validation: Specificity has been validated by WB with recombinant full-size maize Ferredoxin-3 (Fd3) protein.
Applications
- Western blot (1/2,000- 1/10,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western blot of purified maize Ferredoxin-3
The primary antibody was used at 1/2,000 dilution
