anti-GFP antibody, rat monoclonal

Description

Background:

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.

 

Specifications:

Reactivity: Reacts with GFP, EGFP and their fused proteins

Immunogen: Recombinant GFP protein

Isotype: Rat IgG1 kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized

Azide and carrier free.

Storage: Shipped at 4℃ and store at -20℃.

 

Applications

• Western blotting (~1ug/ml)
• Immunoprecipitation
• Immunocytochemistry
• Chromatin Immunoprecipitation (ChIP)
• ELISA

Fig.1 Fluorescent image of COS1 cells due to GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the sams cells were immunostained by using anti-GFP antibody 1A5, followed by Texas Red-conjugated anti-rat IgG (Left). Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence due to GFP

 

Fig.2  Detection of GFP-ZIPK fusion protein  over-expressed in HEK293 cells by Western blotting with antibody 1A5.

(-) HEK293T cell extract without overexpression

( ) GFP-ZIPK protein- overexpressed HEK293T cell extract

 

 

Fig.3  Immunoprecipitation of GST-GFP fusion protein with antibody 1A5.

 

Data Link: UniProtKB/Swiss-Prot P42212 (GFP_AEQVI)

 

References: This antibody has been used in the following publications.

1. Okazaki K. et al. Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. 2012 Oct;33(10):1854-62.  IP
2. Maekawa K. et al. Tissue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin.2015 Sep 17;8:35. ChIP

 

 

Related product: #60-011 anti-GFP antibody, rabbit serum