Description
Background:
NADH-dependent glutamate synthase (GltBD) is involved in glutamate biosynthesis and consists of large subunit (GltB, 168 kDa) and small subunit (GltD, 54 kDa). It is required for non-photorespiratory ammonium assimilation.
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 4 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A/G mix
Immunogen: Purified recombinant cyanobacterium, Leptolyngbya boryana (Plectonema boryanum), glutamate synthase (full-size, no-tag attached)
Reactivity: Reacts with cyanobacteria glutamate synthase, GltB and GltD proteins.
Applications
- Western blot (1/1,000- 1/2,000 dilution)
- ELISA
Fig. 1 Western blot of GltBD protein in cyanobacterium.
Sample: Soluble fraction of Synechocystis sp. PCC6803 extract.
Anti-GltBD antibody was used at 4 µg/ml. As the secondary antibody, goat anti-rabbit IgG antibody HRP-conjugated (ab97051) was used at 1/10,000 dilution.
Molecular mass of GltB (large subunit of NADPH-dependent glutamate synthase) is 168 kDa, GltD (small subunit of NADPH-dependent glutamate synthase) is 54 kDa.
