anti-HCV NS5b protein antibody, monoclonal, FITC conjugated

Description

Background:

Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9,400 bases which encodes a single polyprotein of about 3,010 amino acids (1, 2, 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

 

Applications

• Western blotting
• Immunofluorescence staining
• ELISA

 

Specifications:

Immunogen: A region of NS5b protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli

Conjugate: FITC conjugated, [FITC] / [IgG] = 6.0

Isotype: Mouse IgG2b kappa

Form: 1.3 mg/ml in PBS, 50% glycerol, filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

Storage: Shipped at 4℃ and stored at -20℃

 

Data Link: Swiss-Prot HCV protein

 

References: This antibody (unconjugated) has been produced and used in ref.4.

1. Brass V, Moradpour D, Blum HE. Molecular Virology of Hepatitis C Virus (HCV): 2006 Update. Int J Med Sci 2006; 3:29-34 PMID: 16614739
2. Kato,N. et al. (1990) “Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.” Natl. Acad. Sci. USA 87, 9524-9528 PMID: 2175903
3. Takamizawa, A. et al. (1991) “Structure and organization of the hepatitis C virus genome isolated from human carriers.” Virol.65, 1105-1113 PMID: 1847440
4. Manabe,S. et al. (1994) “Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by N3.” Virology 198, 636-644 PMID: 8291245

 

Related products: #65-066 anti-HCV NS5b antibody,

#67-068 anti-HCV NS5b antibody, biotin conjugated

Fig.1 Western blotting of HCV NS5b protein.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using this antibody. The NS5b protein is detected as a 58-kDa band.

Fig.2 Detection of HCV NS5b protein by immuno-fluorescence antibody staining.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA.
After incubation for 48 hr, the cells were fixed with acetone and HCV NS5b protein was detected by indirect immunofluorescence staining using this antibody.