Description
Heterochromatin protein 1 (HP1) is a major component of heterochromatin and plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1α is encoded by the Chromobox homolog 5 (CBX5) gene located on chromosome 12q13.13. HP1α has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (1).
The product is prepared by immunizing rabbit with the synthetic peptide CEDAENKEKETAKS corresponding to the amino acid sequence 179-191 of human HP1α and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.
Applications
1) Western blot 1/1,000~1/3,000 Fig.1
2) Immunofluorescence staining 1/200~ 1/1,000
Specification
Reactivity: Human. Not tested with other species.
Form: Affinity purified IgG 0.37 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4), 50% glycerol, sterilized by filtration, azide free
Storage: -20°C or long term storage -70°C
Data Link
UniProtKB/Swiss-Prot P45973 (CBX5_HUMAN)
References
- Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID: 17224041
- Kametaka A et al “Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
- Wang F et al “The assembly and maintenance of hetero-chromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 4028-4040 (2006) PMID: 16705157
Fig. Detection of HP1α protein by Western blot.
Lane 1: Crude extract of MCF7 cells
The antiserum was diluted 1,000 fold before use.
