anti-HP1β/CBX1 antibody (rabbit), ChIP grade

$230.00

SKU: 70-223 Research Area: Applications:

Description

Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1β is encoded by the Chromobox homolog 1 (CBX1) gene located on chromosome 17q21.32. HP1β has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (1).

The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1β (3) and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi (Ref. 3).

Applications

1) Western blotting (1/2,000~1/10,000) (Fig. 2)

2) Immunofluorescence staining (Fig1 & Ref. 3)

3) Chromatin immunoprecipitation (ChIP) (Ref. 3)

Specifications

Reactivity: Human and hamster. Expected to react with mouse, chicken, zebra fish, Xenopus and Drosophila orthologs due to the sequence identity of the immunogen.

Form: Affinity purified rabbit IgG, 0.44 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4), 50% glycerol, filter- sterilized, azide free

Storage: -20℃ (long period, -70℃)

Reference:

  1. Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID: 17224041
  1. Kametaka A et al “Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
  1. Wang F et al “The assembly and maintenance of hetero-chromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 4028-4040 (2006) PMID: 167051 57
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