Description
Background:
Ferredoxin-NADP reductase, leaf isozyme 1 (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Subcellular location: Chloroplast
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG fraction, Protein purified.
Validation: Specificity has been validated by WB with recombinant full-size maize FNR1 protein.
Immunogen: Purified recombinant maize leaf FNR1 protein.
Reactivity: Plant leaf FNRs including FNR1 of Maize and Arabidopsis cross reacts with Maize L-FNR2 and L-FNR3, and Arabidopsis L-FNR2. Reacts weakly with root FNR.
Applications
- Western blotting (1/500-1/2,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of L-FNR1 protein
Anti-L-FNR1 antibody was used at 1/500 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg
The molecular masses of mature forms of maize FNR1, FNR2 and FNR3 are 34.97, 35.57 and 34.7 kD, respectively (Ref 2)
