Description
Background:
Ferredoxin-NADP reductase, leaf isozyme 2 (L-FNR2) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Subcellular location: Chloroplast
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with protein A agarose.
Immunogen: Purified recombinant maize leaf FNR2 protein (full size, no tag attached)
Reactivity: Plant L-FNR2 proteins including that of maize and Arabidopsis.
Reacts also with other FNR isoforms, Maize L-FNR1 and L-FNR3, and Arabidopsis L-FNR1
Applications
- Western blotting (1/2,000- 1/50,000 dilution)
- ELISA (assay dependent)
Other applications have not been tested.
Fig.1 Western Blot of L-FNR2 in plant leaf extract
Anti-L-FNR2 antiserum was used at 1/2,000 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- A. thaliana leaf extract (10 μg)
- Z. mays leaf extract (10 μg)
As shown, this antibody cross-reacts with other L-FRN isoproteins
The molecular masses of mature forms of maize FNR1, FNR2 and FNR3 are 34.97, 35.57 and 34.7 kD, respectively
