Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential MED11/22 heterodimer specifically functions in promoting stable PIC formation.
Immunogen: Recombinant His-tagged Med11 protein (1-132) produced in E. coli
Reactivity: Reacts with S. cerevisiae Med11. Not tested with other species.
Form: Protein A affinity purified IgG, 2 mg/ml in PBS, 50% glycerol
Storage: Shipped at 4℃ and stored at -20℃
Western blotting (1/100-1/200).
Not tested for other applications.
Reference: This antibody was described and used in the following publication.
- Takahashi H, Kasahara K, Kokubo T. Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation. Genes Cells.2009 Jan;14(1):53-67.
Fig. Detection of endogenous Med11 by western blotting in whole cell extract of S. cerevisiae,
The antibody was used at at 1/200 dilution.
Blotting was done with wet system after electrophoresis on 15% gel at 15 v overnight. Molecular mass is 13.5 kDa.