anti-Med11 (S. cerevisiae) antibody, rabbit polyclonal, WB

$345.00

SKU: 62-030 Research Area: Applications:

Description

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential MED11/22 heterodimer specifically functions in promoting stable PIC formation.

 

Specifications:

Immunogen: Recombinant His-tagged Med11 protein (1-132) produced in E. coli

Reactivity: Reacts with S. cerevisiae Med11. Not tested with other species.

Form: Protein A affinity purified IgG, 2 mg/ml in PBS, 50% glycerol

Storage: Shipped at 4℃ and stored at -20℃

 

Applications:

Western blotting (1/100-1/200).

Not tested for other applications.

 

Data Link  UniProt Q99278 (MED11_YEAST),  SGD S000004718. MED11.

 

Reference: This antibody was described and used in the following publication.

  1. Takahashi H, Kasahara K, Kokubo T. Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation. Genes Cells.2009 Jan;14(1):53-67.

 

Fig. Detection of endogenous Med11 by western blotting in whole cell extract of S. cerevisiae,

The antibody was used at at 1/200 dilution.

Blotting was done with wet system after electrophoresis on 15% gel at 15 v overnight. Molecular mass is 13.5 kDa.

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