Anti-Nas6/p28 (S. cerevisiae) antibody, affinity purified

Description

The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa.  It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (1).The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP).The RP is further subdivided into lid and base sub-complexes.NAS6 (p28) is a proteasome-interacting protein which acts as a chaperon to assemble the base sub-complex of the 19S RP (2). NAS6 is composed of 226 amino acid residues with calculated molecular masses of 24.4kD (3).

Applications

1) Western blot x 5000 fold dilution (Fig.1)

2) Immunoprecipitation

Not tested for other applications

Specification

Product: Rabbit polyclonal antibody affinity purified with recombinant Nas6

Immunogen: Recombinant yeast Nas6 expressed in E. coli

Form: Purified IgG in PBS, 1 mg/ml BSA, 0.09 % sodium azide, 50% glycerol

Reactivity: S. cerevisiae Nas6, not tested with other species

Storage: -20°C

Data Link

SGD NAS6/YGR232W

References:

1. Hershko A and Ciechanover A “THE UBIQUITIN SYSTEM” Annu Rev Biochem 67: 425-479 (1998) PMID: 9759494
2. Saeki Y et al “Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle” Cell 137:900-913 (2009) PMID: 19446323
3. Hori T et al “cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome” Gene 216:113-122 (1998) PMID: 9714768

Fig.1. Detection of Nas6 in the crude extract of S. cerevisiae by Western blot using this antibody.