Description
Background:
Ferredoxin-nitrite reductase (NiR) is involved in the pathway nitrite reduction (assimilation), which is part of nitrogen metabolism.
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A/G mix
Immunogen: Purified recombinant cyanobacterium (Synechocystis strain 6803) NiR protein (full-size, no-tag attached) expressed in E. coli.
Applications
- Western blot (1/1,000- 1/2,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of NiR protein of Cyanobacterium and Spinacch
Anti-NiR antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Recombinant spinach NiR protein
- Recombinant cyanobacterium (Synechocystis strain 6803) NiR protein
Molecular masses, for spinach NiR, 66 kDa , for Synechocystis NiR, 56 kDa.
