Anti-Pertussis Toxin antibody, rabbit serum

Description

Function: Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecules of S4 (12 kDa). This product was highly purified (>90% pure) from Bordetella pertussis strain Tohama. Cytotoxicity of the PT was confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT.

Applications

1. Western blotting (1/2,000~1/10,000 dilution)
2. ELISA (1/10,000~1/20,000 dilution)
3. Dot blotting (1/2,000~1/10,000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralizing (Assay dependent)

Other applications have not been tested.

Specification

Immunogen: Immunization was Initiated with toxoid and boosted with native toxin

Form: Whole rabbit antiserum added with 0.09% sodium azide

Storage: Shipped at 4°C. Upon arrival, spin down and store at -20°C.

Data Link: Swiss-Prot: Pertussis toxin

Reference:

Alouf JE & Popoff MR (Ed.) The comprehensive Sourcebook of Bacterial Protein Toxins 3rd Ed. Academic Press (2006)

Fig.1. Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western blotting using anti-perussis toxin antibody

1. Culture medium of Bordetera pertussis. SDSPAGE, silver-stained
2. Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained
3. Western blot of culture medium of Bordetera pertussis as in 1
4. Western blot of purified pertussis toxin (10 ng) The toxin consists of five subunits as indicated by S1 to S5.