Anti-PHLDA3 antibody, mouse monoclonal (4B6)

Description

PHLDA3 (Pleckstrin homology-like domain family A member 3) is a small PH (Pleckstrin homology) domain-only protein (127 aa in human) that is highly conserved in vertebrates. Recently PHLDA3 was found to be a p53-regulated repressor of Akt (ref 2) hence it is speculated as a tumor suppressor. PHLDA3 also inhibits anchorage-independent cell growth. PHLDA3 gene is frequently lost in human lung endocrine tumors.

The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Applications

Western blot

Other applications were not tested.

Specification

Immunogen: Synthetic peptide corresponding to N terminal amino acids 1-31 of human PHLDA3

Isotype: Mouse IgG2b kappa

Form: Purified monoclonal antibody (IgG) 1 mg/ml in PBS, 50% glycerol, filter-sterilized

Specificity: Specific to human PHLDA3.  Not tested with other species.

Storage: -20°C, for long term storage -70°C

Data Link

UniProtKB/Swiss-Prot Q9Y5J5 (PHLA3_HUMAN)

References:  This antibody has been used in Ref. 2

1. Frank D et al (1999) “A novel pleckstrin homology-related gene family defined by Ip1/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting” Genome 10:1150-1159 PMID: 10594239
2. Kawase T et al (2009) “PH domain-only PHLDA3 is a novel p53-regulated repressor of Akt” Cell 136: 535-550 PMID: 19203586

Fig.1 Western blot was performed using the antibody 4B6 to detect PHLDA3 protein expression. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).