Anti-POGZ antibody, rabbit polyclonal

Description

Description

POGZ (pogo transposable element-derived protein with zinc finger domain, 1,410 aa, 155 kDa) plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 (HP1 alpha) it plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.

 

Applications

1. Western Blot (1/1,000 dilution)
2. Immunofluorescence staining (1/500 dilution)
3. Immunoprecipitation (assay dependent)

Not tested for other applications

 

Specification

Immunogen: Recombinant protein expressing 1-562 amino acids of human POGZ.

Reactivity: Reacts specifically with human and mouse POGZ. Not tested in other species.

Form: Rabbit antiserum. No sodium azide added. Sterilized by filtration through 0.22 µm membrane.

Storage: Ship at 4°C or -20°C. Store at -20°C. Avoid repeated freeze-thaw cycle.

 

Data links: Entrez Gene: 23126 Human

                   SwissProt: Q7Z3K3 Human

 

Reference:  This antibody was used in the following article.

Nozawa R.S et al (2010) Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nat. Cell Biol. 12:719-727

PubMed: 20562864

Fig.1 Western blot of POGZ

Lane 1: Crude extract of HeLa cells transfected with control siRNA

Lane 2: Crude extract of HeLa cells transfected with POGZ specific siRNA

The antibody was used at 1/1,000 dilution.

Specificity of the antibody to POGZ is confirmed by the disappearance of the POGZ band in the cells treated with the POGZ specific siRNA (lane 2)

Fig.2 Immunofluorescence staining of POGZ.

Paraformaldehyde fixed HeLa cells were stained with Hoechst 33342 and anti-POGZ antibody. The antibody was used at 1/500 dilution. Scale bar, 10 μm

Fig. 3 Immunofluorescence staining of POGD on chromosomes.

Chromosome spreads were prepared from HeLa cells and stained with Hoechst 33342 and anti-POGZ antibody. The antibody was used at 1/500 dilution. Scale bar, 10 μm