Anti-Rad52 (human) antibody, antigen affinity purified

Description

Human Rad52 protein is a functional and structural homolog of yeast Rad52 protein which plays a major role in genetic recombination and recombination repair by mediating strand annealing between homologous DNA strands (Ref.1). Rad52 functionally and physically interacts with Rad51 in recombination processes. It also interacts with oncogenes and tumor suppressors for the maintenance of genome stability (Ref.1).

The product was prepared by immunizing rabbit with the full-size recombinant Rad52 protein expressed in E. coli and affinity-purified with the recombinant Rad52.

Using this anti-serum, Rad52 protein (46 kD) was detected by Western blotting (Fig. 1) at 46 kD position. This antibody was successfully used for immunoprecipitation assay (Fig. 1).

Applications

• Western blot (x 2,000)
• Immunoprecipitation
• Detection of foci formation by indirect immunofluorescence method

Specification

Reactivity: Reacts with human and mammalian Rad52 protein

Form: 1 mg/ml PBS (20mM Na-phosphate pH7.5, 0.15M NaCl), 50% glycerol

Storage: -20°C

Data Link: Swiss-Prot P43351

Reference

Friedberg EC et al DNA Repair and Mutagenesis 2^nd ed.,

ASM Press (2006)

Fig.1 Western blot and immunoprecipitation of Rad52

Lane1: 1 ng/lane purified hRad52 protein (catalog # 10-003)

Lane2: immunoprecipitation sample (MCF’)