Description
Background:
Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
RFA encodes 621 amino acids with molecular mass of 70,348.
Specifications:
Reactivity: S. cerevisiae Rfa1 protein.
Immunogen: Recombinant Rfa1 protein
Form: Rabbit antiserum added with 0.05% sodium azide.
Storage: Shipped at 4℃ and store at -20℃.
Applications:
Western blotting (1/2,000 dilution)
Immunoprecipitation (1/200)
Data Link: UniProtKB P22336 (RFA1_YEAST), SGD S000000065 RFA1 / YAR007C
Fig. Detection of endogenous Rfa1 protein in whole cell extract of S.cerevisiae.
The extract of strain BY4741 (10 μg) was separated on SDS-PAGE 10-20% gradient gel. The anti-Rfa1 antibody was used at 1/2,000 dilution. As the second antibody, HRP-conjugated goat anti-rabbit IgG (ab97051) was used at 1/10,000 dilution
