Anti-Rhp51 /Rad51 (S. pombe) antibody, rabbit polyclonal, ChIP grade

Description

Background:

Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes, which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands.

 

Specifications:

Reactivity: Schizosaccharomyces pombe. Specificity has been validated with rhp51 mutant for WB (Fig.1)

Immunogen: Purified recombinant full-length Rhp51 protein

Form: Rabbit antiserum added with 0.09 % sodium azide

Storage temmperature: Shipped at 4℃ and store at -20℃

 

Applications:

• Western blotting (1/ 2,000~1/5,000) Fig.1
• Immunoprecipitation (1/100-1/500)
• Chromatin Immuno-Precipitation (Assay dependent)
• Immunofluorescence staining (1/500 dilution). Fig. 2

 

Data Link UniProtKB/Swiss-Prot P36601 (RAD51_SCHPO)

 

Fig.1 Western blot analysis of Rhp51 in the whole cell extracts.

M: Molecular size markers (kD)

Lane 1: Wild-type strain

Lane2: Rhp51 deletion mutant strain

Note that Rhp51 band is detected in lane 1, wild-type extract, but is absent in lane 2, rhp51 mutant extract.

 

Fig. 2 Rhp51 foci formation observed after DNA damage: S. pombe cells without or with MMS (0.025%) treatment for 1 h were processed for indirect immunofluorescence staining  with anti-Rhp51 antibody (1/500 dilution). The Rhp51 foci formation is induced by DNA-damage treatment with MMS.

 

References: This product has been used in the following publications

1. Akamatsu Y et al. Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5. WB, IP (S. pombe)

2. Kibe T et al. Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer. Nucleic Acids Res.2003 Sep 1;31(17):5054-63. ChIP (S. pombe)

3. Lambert S et al “Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier” Cell 121: 689-702 (2005) PMID: 15935756 IF (S. pombe)

4. Morishita T et al” Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates” Mol Cell Biol 25: 8074-8083 (2005) PMID: 16135799  IF (S.pombe)

5. Haruta N et al “The Swi5-Sfr1 complex stimulates Rhp51/Rad51-and Dmc1-mediated DNA strand exchange in vitro”Nat Struc Mol Biol 13: 823-830 (2006) PMID: 16921379 WB, IP (S. pombe)

6. Akamatsu Y et al. Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes. EMBO J.2007 Mar 7;26(5):1352-62. IF (S. pombe)

7. Polakova S et al. Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation. PLoS Genet.2016 Jun 15;12(6):e1006102. IF (S. pombe)