Description
Background:
Ferredoxin-NADP reductase (FNR) isoproteins of plant roots play a key role in redox homeostasis of NADPH / NADP+ and donation of reducing equivalence to ferredoxin. R-FNR2 is major form of R-FNR and involved in reduction and detoxication of nitrite in root.
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, Protein A-affinity purified from rabbit antiserum
Immunogen: Purified recombinant maize root-FNR protein (full size, no tag attached)
Reactivity: Plant root FNR proteins (R-FNR1 and R-FNR2) including Arabidopsis and Maize. The antibody also reacts with leaf FNRs.
Applications
- Western blotting (1/1,000- 1/30,000 dilution)
- ELISA (assay dependent)
Other applications have not been tested.
Fig.1 Detection of Arabidopsis R-FNR1 and R-FNR2 isoproteins by Western blot with anti-R-FNR antibody
Extracts from wild type strain Arabidopsis Col (1 and 3), mutant rfer1 (2) and rfnr2-2 (4) grown under 0.2 mM nitrate for 7 days were analyzed by western blotting. Anti-R-FNR antibody was used at 1/2,000 dilution. Note that R-FNR2 is dominant form in wild-type roots.
Wild type produces both R-FNR1 and R-FNR2.
Mutant rfnr1 produces R-FNR2 and mutant rfr2 produces R-FNR1.
