Description
Background:
RRM1 (Ribonucleoside-diphosphate reductase 1) catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides and provides the precursors necessary for DNA synthesis. RRM1 consists of 797 amino acids with molecular mass of 90,833.
Specifications:
Reactivity: Xenopus, human, mouse, rat, hamster
Immunogen: Recombinant His-tagged RRM2 (182-717 amino acids) of Xenopus laevis.
Form: IgG (Protein A affinity purified) 2 mg/ml in PBS, 50% glycerol, Filter-sterilized
Storage: Shipped at 4℃ and aliquot and store at -20℃
Applications:
Western blotting (1/1,000-1/2,000 dilution)
Immunofluorescence staining (1/100 dilution)
Data Link UniProtKB/Swiss-Prot Q6GM88 (Q6GM88_XENLA)
Reference: This product was used in the following publication.
- Takada S. et al. Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts. Mol Biol. Cell 11: 4173-4187 (2000) PMID: 11102516 WB, IF (Xenopus)
Fig.1 Detection of endogenous RRM1 protein in crude extrcats of mammalian cells and Xenopus eggs.
The antibody was used at 1/1,1000 dilution
- Xenopus lavaes eggs 2. HeLa cells 3. CHO cells
- HeLa cells 5. MCF7 cells 6. NIH3T3 cells
- CHO cells
