Anti-Streptococcus NADase Inhibitor Antibody, Rabbit Polyclonal

Description

Function:

NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. NADase inhibitor forms a complex with NADase in cells and only NADase is secreted into culture medium.

Applications

Western blotting (1/3,000 dilution)

Immunoprecipitation

ELISA

Specification

Immunogen: Purified recombinant His-tagged NADase inhibitor of S. dysgalactiae expressed in E. coli

Reactivity: NADase inhibitor of Streptococcus

Form: Undiluted anti-serum added with 0.09 % sodium azide

Storage: Shipped at 4°C or at -20°C. Upon arrival, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.

Data Link:

UniProt/U3TL99 (Streptococcal NAD glycohydrolase inhibitor)

Reference:

This antibody was used in the following publications:

Kimoto H et al “Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor ”J Biol Chem 281: 9181-9189 (2006) PMID: 16380378

Figure. Identification of NADase inhibitor in crude extracts by western blotting with anti NADase inhibitor antibody. The antibody was used at 1/3,000 dilution. (1) Cell lysate of S. dysgalactiae (2) Cell lysate of E. coli overexpressing His-tagged NADase NADase inhibitor (3) Culture supernatant of S. dysgalactiae (4) Molecular size markers.