anti-Taf1/TFIIEa (S. cerevisiae) antibody, rabbit serum

Description

Background:

Tfa1 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Taf14 consists of 482 amino acids with molecular mass of 54,742 Da.

Specifications:

Immunogen: Recombinant His-tagged Tfa1 protein (1-482 aa) produced in E. coli

Reactivity: S. cerevisiae Tfa1 protein. Not tested with other species

Form: Whole antiserum added with 0.1% sodium azide

Storage: Shipped at 4℃and stored at -20℃ for long period.

Applications:

• Western blotting (1/2,000). Not tested for other applications.

Data Link: UniProt P36100 (T2EA_YEAST),   SGD S000001511 TFA1 / YKL028W

Figure. Detection of endonenous Tfa1 in whole cell extract of S. cerevisiae by Western blotting, using the anti-Tfa1 antibody.

The antibody was used at 1/2,000 dilution.

As second antibody, HRP-conjugated goat anti-rabbit IgG was used at 1/10,000

The apparent molecular mass (~65 kDa) is larger than calculated mass of 55 kDa, which may be due to its highly acidic cluster at C-terminal 106 amino-acids (Asp/Glu rich).

Reference: This antibody was described and used in the following publication.

1. Takahashi H. et al. Saccharomyces cerevisiaeMed9 comprises two functionally distinct domains that play different roles in transcriptional regulation. Genes Cells. 2009 Jan;14(1):53-67. doi: 10.1111/j.1365-2443.2008.01250.x. WB