anti-Taf3(S. cerevisiae) antibody, rabbit serum

Description

Background:

Taf3 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Taf3 consists of 353 amino acids with molecular mass of 40,296 Da.

Specifications:

Immunogen: Recombinant His-tagged Taf3 protein produced in E. coli

Reactivity: Reacts with S. cerevisiae Taf3.  Not tested with other species.

Form: Whole antiserum added with 0.1% sodium azide

Storage: Shipped at 4℃ and stored at -20℃

Applications: Western blotting.  Not tested for other applications.

Data Link  UniProt  Q12297  (TAF3_YEAST),   SGD S000005932 TAF3 / YPL011C

Reference: This antibody was described and used in the following publication.

1. Takahata K. et al. Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation. Mol Cell Biol.2004 Apr;24(8):3089-99.