Anti-Taf7 (S. cerevisiae) antibody, rabbit polyclonal

Description

TAf7 transcription coactivator interacts with the largest subunit of the TFIID protein complex. The TFIID complex binds to the TATA box in promoters targeted by RNA polymerase II. TAF7 is responsible for the recruitment of BDF1 to TATA element containing promoters.

Applications

Western blot 1:500 dilution

Not tested for other applications.

Specifications

Immunogen: Recombinant His-tagged Taf7 protein (1-214 aa) produced in E. coli

Reactivity: S. cerevisiae Taf7 protein. Not tested with other species

Form: Whole antiserum added with 0.1% sodium azide

Shipped at 4°C and stored at -20°C

Data Link

UniProt Q05021 (TAF7_YEAST) SGD S000004840 TAF7 / YMR227C

Reference

Takahata S. et al. Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation. Mol Cell Biol. 2004 Apr; 24(8):3089-99.

Fig.1 Detection of endonenous Taf7 by Western blot using the anti-Taf7 antibody. The antibody was used at 1/500 dilution. Blotting was done with a wet system.