Anti-Tem1 (S. cerevisiae) antibody, affinity purified

Description

Tem1 is a low-molecular-weight GTP-binding protein (GTPase) which is required for the termination of M phase in cell division. The defect of Tem1 was lethal, and the Tem1-defective cells were arrested at telophase with high H1-kinase activity, indicating that Tem1 is required to exit from M phase. The defect of Tem1 was suppressed by a high dose of cdc15, which encodes a protein kinase. Tem1 functions upstream of cdc15 kinase and may be required to activate the cdc15 protein kinase pathway. A cascade consisting of Tem1 and kinases act to terminate mitosis.

This product is a rabbit polyclonal antibody affinity purified with the immunogen after adsorption of anti-GST antibody with GST－affinity column.

Applications

1) Western blot 1/250~1/500

2) Immunoprecipitation

Specification

Immunogen: GST-full length Tem1 fusion protein expressed in E. coli

Form: Affinity purified IgG (unknown concentration) in PBS, 1 mg/ml BSA as carrier, 0.09 % sodium azide, 50% glycerol

Reactivity: S. cerevisiae Tem1, not tested with other species

Storage: Ship at 4°C and stored at -20°C

Data Link

SGD TEM1/YML064C

References:

1. Shirayama M et al “The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase.” Mol Cell Biol 14, 7476-7482 (1994) PMID: 7935462
2. Shou W et al “Exit from mitosis is triggered by Tem1-dependent release of protein phosphatase Cdc14 from nucleolar RENT complex.” Cell 97:233-244 (1999) PMID: 10219244
3. Lippincott J et al “The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis.” J Cell Sci 114:1379-1386 (2001) PMID: 11257003

Fig.1. Detection of Tem1 (28kD) in the crude extract of S. cerevisiae by Western blot using this antibody.