Anti-Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody, rabbit serum

Description

Description

Vero toxin 1 (VT1) is produced by Vero toxin1-producing E. coli (VTEC) and has lethal activity to Vero cells. The primary structure of VT1 is identical or nearly identical to Shiga toxin (Stx) produced by Shigella dysenteriae serotype 1 and also called Slt 1 (Shiga-like toxin 1). VT1 is composed from one A subunit and five B subunits. Some E. coli strains produce both Slt1 and Slt2, and they share sequence identity of 55 %, but they are immunologically distinct.

To express the activity of VT/Stx, interaction with specific receptor Gb3 is indispensable. VT/Stx removes the 4324th adenine of 28S RNA of ribosome, inhibits protein synthesis and causes cell death. After invasion into cell subunit A is cut by furin to give A1 and A2. A1 is a catalytic fragment, and A2 is required for holo-enzyme formation by combining subunit B.

Applications

1) Western blot (2,000 fold dilution) (Fig. 1)

2) Immunoprecipitation (Fig. 2)

3) ELISA

Other applications have not been tested.

Specification

Immunogen: Initial immunization by VT1 toxoid and booster by VT1 toxin.

Reactivity: VT1 of E. coli VTEC strain and Shiga toxin of Shigella dysenteriae 1.

Form: Rabbit antiserum added with 0.09% sodium azide.

Storage: Ship at 4°C. Upon arrival, centrifuge, aliquot and store at -20°C

Data Link  GenBank  M16625　Shiga-like toxin I subunit A and subunit B

UniProtKB/Swiss-Prot Q9FBI2 Shiga toxin subunit A

UniProtKB/Swiss-ProtQ7BQ98 Shiga toxin subunit B

Fig.1.Detection of VT1 by Western blot with anti-VT1 antibody.

1. SDS-PAGE of culture medium of VTEC, stained with CBB
2. SDS-PAGE of crude extracts of VTEC cells, stained with CBB
3. Western blot of culture medium of VTEC
4. Western blot of crude extracts of VTEC cells.

Ani-VT1 antibody was used at 1/2,000 dilution