anti-yeast Sua7p antibody, rabbit serum

Description

Background:

The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance. Sua7p is the TFIIB of budding yeast and is composed of 346 amino acid residues (aa).

Specifications:

Reactivity: S. cerevisiae Sua7 / TFIIB protein

Immunogen: Rcombinant His-tagged full-size Sua7 protein

Form: 0.1% sodium azide added to the antiserum

Storage temperature: Ship at 4℃and store at -20℃

Applications:

• Western blotting. (1/1,000~1/5,000)
• Immunoprecipitation
• Chromatin Immuno-Precipitation
• ELISA

Data Link SGD SUA7/YPR086W

Fig.1 Detection of endogenous Sua7 protein by Western blotting.

M: protein size marker in kDa; Lane1, Crude extract of S. cerevisiae

The antiserum was diluted 5000 fold before use.

References: This antibody has been used in the following publication.

1. Kasahara K. et al. Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters bymasking a nucleosome-free region. Nucleic Acids Res.2011 May;39(10):4136-50. PMID: 21288884 ChIP