Description
Background:
The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time, it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf4p is one of the subunits of TFIID and in the case of budding yeast, it is composed of 388 amino acid residues (aa). This protein contains histone folds in its interior and forms TAF octamer with Taf6p, Taf9p and Taf12p.
Specifications:
Reactivity: S. cerevisiae Taf4 protein.
Immunogen: Recombinant His-tagged N-terminal domain of Taf4p protein (1-200aa)
Form: Whole antiserum added with 0.1% sodium azide added to the antiserum.
Storage temperature: Ship at 4℃ and store at -20℃
Applications
- Western blotting. (1/1,000~1/5,000 dilution)
Fig.1 Detection of Taf4p by Western blotting
Lane 1: Extract of budding yeast
The antiserum was diluted 5,000 fold before use.
The molecular mass of Taf4 is 48 kDa.
Data Link SGD S000004607/overview
References: This antibody was described and used in the following publication.
- Takahata S et al “Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation” Mol Cell Biol 24: 3089-3099 (2004) PMID: 15060133 WB
Reviews
There are no reviews yet.