KOD SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using SYBR® Green I and based on the 3’→5′ exonuclease deficient KOD DNA Polymerase. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup, and improves the reproducibility of experiments.This product was optimized to be highly efficient and robust in the SYBR® Green assay.
The reagent includes the following components for 40 reactions (QKD-201T) or 200 reactions (QKD-201), 50 µL total reaction volume:
KOD SYBR® qPCR Mi 1.67 mL × 3
50x ROX reference dye 250 µL
KOD SYBR® qPCR Mix 1 mL
50x ROX reference dye 50 µl