Pfu DNA polymerase (with dNTPs)

Description

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa same as that of the natural Pfu DNA polymerase. Pfu DNA polymerase is thermostabe and has low error rates. It is suitable for PCR and primer extension reactions that require high fidelity synthesis. Pfu DNA polymerase-generated PCR fragments are blunt-ended. Applications: cloning, DNA expression, site-directed mutagenesis