ReverTra Ace qPCR RT Kit

$207.30

High Efficient Reverse Transcription Kit for Real-time PCR

SKU: FSQ-101 Research Area:

Description

ReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase “”ReverTra Ace™”” and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min.
ReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.

 

<FEATURES>
The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.

 

<APPLICATIONS>
Reverse transcriptation for real-time PCR

ReverTra Ace™ qPCR RT Kit
5 x RT Buffer 400 µL
Enzyme Mix 100 µL
Primer Mix 100 µL
Nuclease-free water 1000 µL × 2″