ReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase “”ReverTra Ace™”” and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min.
ReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.
Reverse transcriptation for real-time PCR
ReverTra Ace™ qPCR RT Kit
5 x RT Buffer 400 µL
Enzyme Mix 100 µL
Primer Mix 100 µL
Nuclease-free water 1000 µL × 2″