The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimidefunctionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel. Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.
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