CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. Within the cell, the CD40 molecule acts as a transmembrane signal transducer that leads to activation of intracellular kinases and transcription factors.
The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and the IgG was purified under mild conditions by propriety chromatography processes.
Immunohistochemistry (acetone-fixed section; indirect immuno-staining)
Stimulation of B cell proliferation in the presence of IL4
Isotype: Mouse IgG1κ
Immunogen: Recombinant extracellular domain of CD40
Form: 1mg/ml in PBS, 50% glycerol, filter-sterilized
Storage: Shipped at 4°C and stored at -20°C
Inui S et al (1990) Eur J Immunol 20: 1747-1753 PMID: 1698631
Yasui T et al (2002) Int Immunol 14: 319-329 PMID: 11867568
Ishida I et al (2003) Int Immunol. 15: 1027-1034 PMID: 12882840
Fig.1 B cell-specific expression of h (human) CD40 in transgenic mice (ref.2). Splenocytes from m (mouse) CD40+/-, mCD40-/- and hCD40 wild type/mCD40-/- mice were stained with monoclonal antibodies against mCD40, B220 and hCD40 (5C3) and analyzed by flow cytometry. hCD40 molecules were expressed specifically on B220+ B cells.