Anti-Cdc37 (S. cerevisiae) Antibody, Rabbit Serum

$465.00

SKU: 62-302 Research Area: Applications:

Description

Cdc37 was initially identified as a cell division cycle control protein of Saccharomyces cerevisiae (1) and was later found to have a much broader role as a molecular chaperone required for folding of protein kinases (2). It forms a complex with Hsp90 and a variety of protein kinases and is thought to play a critical role in directing Hsp90 to its target kinases (3). Cdc37 has a molecular weight of 58.4 kD.

 

Applications

Western blot (2,000 fold dilution)

Immunoprecipitation

Indirect immuno-staining

Not tested for other applications.

 

Specification

Product: Rabbit polyclonal antibody

Immunogen: Recombinant yeast Cdc37 expressed in E. coli

Form: Antiserum with 0.09% sodium azide

Reactivity: S. cerevisiae Cdc37, not tested with other species

Storage: -20°C. For long term storage, -70°C

 

Data Link

SGD CDC37/YDR168W

References

Reed SI “The selection of S. cerevisiae mutants defective in the start event of cell division” Genetics 95: 561-577 (1980) PMID: 7002718

 

Kimura Y et al “Cdc37 is a molecular chaperone with specific functions in signal transduction” Genes Dev 11: 1775-1785 (1997) PMID: 9242486

 

Stepanova L et al “Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4” Genes Dev 10: 1491-1502 (1996) PMID: 8666233

 

 

Fig. 1 Detection of Cdc37 protein in the crude extract of S. cerevisiae by Western blot using this antibody. Lane 1: x 1000 dilution; Lane 2: x 5000 dilution; Cdc37 protein has a molecular weight of 58.4 kD, but appeared as a 68 kD band in SDS-PAGE.

 

Title Type Size
62-302_03222018 application/pdf 184 KB

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